中国兽医科学2026，Vol.56Issue(3)：285-295,11.DOI:10.16656/j.issn.1673-4696.2026.0039
弓形虫含MFS结构域蛋白TGME49_226020的基本生物学功能研究
Studies of basic biological function of the MFS domain-containing protein encoded by Toxoplasma gondii TGME49_226020 gene
摘要
Abstract
In order to explore the basic biological function of the major facilitator superfamily(MFS)domain-containing protein encoded by Toxoplasma gondii TGME49_226020 gene,CRISPR-Cas9 technology combined with minimum auxin-inducible degron system was used to construct conditional knockout strains on T.gondii RH-TIR1 and Pru-TIR1 strains,respectively.Indirect immunofluorescence assay showed that TGME49_226020 protein was localized in cytoplasm and was successfully degraded after adding indole acetic acid(IAA).The ability of plaque-forming,invasion,intracellular replication was significantly decreased by successful degradation of TGME49_226020,but without impact on egress ability.The organelle stability test showed that the degradation of TGME49_226020 had no significant effect on the organelle stability of T.gondii.The bradyzoite transformation ability of RH-TIR1-TGME49_226020-mAID strain and Pru-TIR1-TGME49_226020-mAID strain was significantly reduced compared with the corresponding RH-TIR1 wild strain and Pru-TIR1 wild strain after adding IAA.Conclusion:considering that TGME49_226020 is a transport protein containing MFS domain,the degradation of TGME49_226020 protein may affect the normal operation of several metabolic transport pathways in T.gondii,and the underlying mechanism warrants further studies.关键词
弓形虫/条件性缺失/MFS结构域/生长素诱导降解系统Key words
Toxoplasma gondii/conditional knockdown/MFS domain/auxin-inducible degradation system分类
农业科技引用本文复制引用
马志雅,李婷婷,李文硕,王萌,朱兴全,张念章..弓形虫含MFS结构域蛋白TGME49_226020的基本生物学功能研究[J].中国兽医科学,2026,56(3):285-295,11.基金项目
西藏自治区科技计划项目(QYXTZX-RKZ2024-03-3) （QYXTZX-RKZ2024-03-3）
山西农业大学高层次人才科研专项(2021XG001) （2021XG001）
甘肃省科技重大专项计划项目(23ZDNA007) （23ZDNA007）
兰州市科技计划项目(2025-2-97) （2025-2-97）
