中国兽医科学2026，Vol.56Issue(3)：304-311,8.DOI:10.16656/j.issn.1673-4696.2026.0032
非洲猪瘟病毒p30、p54和p72蛋白多表位抗原的制备及其免疫原性评价
Preparation and immunogenicity evaluation of multi-epitope antigens of African swine fever virus p30,p54,and p72 proteins
摘要
Abstract
This study aimed to prepare multi-epitope antigens of African swine fever virus(ASFV)and evaluate its application in vaccine.We designed a prokaryotic recombinant plasmid expressing the molecular chaperone TF16 and ASFV multi-epitope antigen,which can solubly express ASFV-pBT multi-epi-tope recombinant protein.After purification and identification,mice were immunized and their immuno-genicity was evaluated,providing a test basis for the research of subunit vaccines against African swine fever.The results showed that ASFV-pBT can be solubly expressed in Escherichia coli and specifi-cally recognized by ASFV antibody positive serum.The ASFV-pBT immunized group achieved serum antibody titers up to 1∶32 000,and ASFV-pBT could effectively activate the proliferation of T lymphocytes in mice.Compared with the negative control group,mice in the ASFV-pBT group exhibited significantly in-creased proportions of CD4+and CD8+T lymphocytes(P＜0.001)and IFN-γ and IL-4 cytokine levels were markedly elevated(P＜0.001).In vivo and in vi tro double validation showed that ASFV-pBT recombinant protein possessed strong immunogenicity,inducing robust humoral and cellular immune respons es,thereby laying reference for ASFV epitope vaccine development.关键词
非洲猪瘟病毒/多表位蛋白/触发因子/免疫原性Key words
African swine fever virus/multi-epitope protein/trigger factor/immunogenicity分类
农业科技引用本文复制引用
杨锐,茹毅,蒋成辉,常艳燕,郝荣增,张越,杭洁..非洲猪瘟病毒p30、p54和p72蛋白多表位抗原的制备及其免疫原性评价[J].中国兽医科学,2026,56(3):304-311,8.基金项目
兰州市青年科技人才创新项目(2023-QN-3) （2023-QN-3）
江西中医药大学博士启动基金(2024-5152500110) （2024-5152500110）
