中国兽医科学2026，Vol.56Issue(3)：356-361,6.DOI:10.16656/j.issn.1673-4696.2026.0034
非洲猪瘟病毒B169L蛋白多克隆抗体制备
Preparation of polyclonal antibodies against African swine fever virus B169L protein
摘要
Abstract
B169L is a membrane protein encoded by the African swine fever virus(ASFV),and its biological function remains unclear.To facilitate the investigation of its potential roles,a polyclonal antibody against B169L was generated in this study.Bioinformatic analysis predicted that B169L contained two transmembrane domains and a signal peptide,suggesting its localization in the endoplasmic reticulum.The truncated B169L protein lacking transmembrane regions was expressed and purified using an Escheri-chia coli prokaryotic expression system,and used to immunize rabbits for polyclonal antibodies production.Western-blot analysis demonstrated that the obtained antibodies specifically recognized the B169L protein expressed in ASFV-infected cells.Collectively,these results indicated that a specific polyclonal antibody against B169L was successfully prepared,providing an essential tool for future studies on the structure and function of the ASFV membrane protein B169L.关键词
非洲猪瘟病毒/B169L蛋白/原核表达/多克隆抗体Key words
African swine fever virus/B169L protein/prokaryotic expression/polyclonal antibodies分类
农业科技引用本文复制引用
武正前,周万卉,黄秀梅,邓宠贤,张旭辉,朱向涛,李丹,杨文萍,郑海学..非洲猪瘟病毒B169L蛋白多克隆抗体制备[J].中国兽医科学,2026,56(3):356-361,6.基金项目
国家自然科学基金青年项目(32202780) （32202780）
甘肃省科技厅青年科技基金项目(23JRRA552) （23JRRA552）
甘肃省高校教师创新基金项目(2025B-064) （2025B-064）
动物疫病防控全国重点实验室开放基金项目(SKLADCPKFKT202408) （SKLADCPKFKT202408）
国家生猪技术创新中心先导科技项目(NCTIP-XD/C03) （NCTIP-XD/C03）
国家生猪产业技术体系项目(CARS-35,CARS-39-13) （CARS-35,CARS-39-13）
中国农业科学院兰州兽医研究所基本科研业务项目(No.1610312022005) （No.1610312022005）
