中国药房2026，Vol.37Issue(6)：727-732,6.DOI:10.6039/j.issn.1001-0408.2026.06.06
炮制前后的栀子-黄芩药对中5种活性成分含量及指纹图谱研究
Study on the contents of five active components and fingerprint of Gardenia jasminoides-Scutellaria baicalensis herb pairs before and after processing
摘要
Abstract
OBJECTIVE To establish the method for determining the contents of five active components in raw and charred herb pairs of Gardenia jasminoides-Scutellaria baicalensis,construct their fingerprints,and investigate the effects of G.jasminoides processing on chemical constituents in the herb pairs.METHODS The HPLC method was used to determine the contents of genipin 1-β-D-gentiobioside,geniposide,crocin Ⅰ,crocin Ⅱ and baicalin in ten batches of raw G.jasminoides-S.baicalensis herb pairs and ten batches of charred G.jasminoides-S.baicalensis herb pairs.Using the same HPLC method,chromatographic fingerprints for the ten batches of raw herb pairs and ten batches of processed herb pairs were established with the Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine(2012 edition).Principal component analysis and orthogonal partial least squares-discriminant analysis were performed.RESULTS Compared with the raw G.jasminoides-S.baicalensis herb pair,the average content of genipin 1-β-D-gentiobioside in the charred G.jasminoides-S.baicalensis herb pair increased by 12.65%;while the average contents of geniposide,crocin Ⅰ,crocin Ⅱ and baicalin decreased by 7.86%,60.62%,62.07%and 0.15%,respectively.Chromatographic fingerprints of ten batches of raw herb pairs and ten batches of processed herb pairs shared 18 common peaks with similarities ranging from 0.997 to 1.000 and 0.988 to 1.000,respectively.Five common peaks were identified:genipin 1-β-D-gentiobioside(peak 2),geniposide(peak 3),crocin Ⅰ(peak 6),crocin Ⅱ(peak 9)and baicalin(peak 10).Principal component analysis and orthogonal partial least squares-discriminant analysis results showed that the raw G.jasminoides-S.baicalensis herb pair and the charred G.jasminoides-S.baicalensis herb pair could be clearly distinguished.Variable importance in the projection(VIP)values for crocin Ⅰ and crocin Ⅱ were both greater than one.CONCLUSIONS A method has been successfully developed for the determination of five active components,including genipin 1-β-D-gentiobioside,in the G.jasminoides-S.baicalensis herb pair,and its fingerprint has been drawn.Processing is found to increase the content of genipin 1-β-D-gentiobioside,while decreasing the levels of geniposide,crocin Ⅰ,crocin Ⅱ and baicalin in the herb pair.Furthermore,crocin Ⅰ and crocin Ⅱ could serve as potential quality markers for the quality control of this herb pair.关键词
炮制/生栀子/焦栀子/黄芩/药对/含量测定/指纹图谱Key words
processing/raw Gardenia jasminoides/charred G.jasminoides/Scutellaria baicalensis/herb pair/content determination/fingerprint分类
医药卫生引用本文复制引用
李玉,祁俊,吴萍..炮制前后的栀子-黄芩药对中5种活性成分含量及指纹图谱研究[J].中国药房,2026,37(6):727-732,6.基金项目
全国老药工传承工作室建设项目(No.国中医药人教函[2024]255号) （No.国中医药人教函[2024]255号）
