中国药房2026，Vol.37Issue(6)：733-739,7.DOI:10.6039/j.issn.1001-0408.2026.06.07
硬尖神香草抗支气管哮喘气道重塑的质量标志物研究
Study on quality markers of Hyssopus cuspidatus against airway remodeling in bronchial asthma
摘要
Abstract
OBJECTIVE To identify the quality markers(Q-Markers)of Hyssopus cuspidatus against airway remodeling in bronchial asthma(referred to as"asthma"),and to provide a reference for the quality control research of H.cuspidatus based on pharmacodynamic activity.METHODS Potential active components and action targets of H.cuspidatus were screened by network pharmacology method.Using human airway smooth muscle cells(HASMCs)as objects,airway remodeling cell model was induced by platelet-derived growth factor-BB(PDGF-BB);validation test was then performed for anti-asthmatic effects of H.cuspidatus and the potential active components.HPLC method was employed to establish the fingerprints of 14 batches of H.cuspidatus samples,and chemometric analysis was also conducted.Combined with the results of pharmacodynamic experiments and fingerprint analysis,the Q-Markers of H.cuspidatus against airway remodeling in asthma were determined.RESULTS&CONCLUSIONS Network pharmacology analysis showed that the potential active components of H.cuspidatus against asthma might be flavonoids and phenolic acids such as luteolin,quercetin and rosmarinic acid,and the core anti-asthmatic targets were interleukin-6,mitogen-activated protein kinase,etc.In vitro experimental results confirmed that 25,50,100 μg/mL of H.cuspidatus,as well as neochlorogenic acid(80 μmol/L),acacetin(80 μmol/L),salvianolic acid B(40 μmol/L)and quercetin-3-O-β-D-glucuronide(80 μmol/L),significantly reduced the cell viability induced by PDGF-BB,inhibited cell proliferation,migration,and the phosphorylation level of extracellular signal-regulated protein kinase 1/2,decreased the levels of interleukin-6,tumor necrosis factor-α and reactive oxygen species,and generally arrested cells in the G0/G1 phase(P＜0.05).Fingerprint analysis showed that there were 27 common peaks in the fingerprints of the 14 batches of H.cuspidatus samples,with 15 compounds(including luteolin)identified,and the similarities of fingerprints were all greater than 0.8.The 14 batches of samples could be divided into three categories:S1-S7 as one category,S8-S13 as one category,and S14 as one category.The variable importance in the projection values of rosmarinic acid,chlorogenic acid,caffeic acid,salvigenin,luteolin,ferulic acid,quercetin-3-O-β-D-glucuronide,and the components corresponding to peaks 5 and 8 were greater than 1,indicating they were potential differential markers affecting quality.Integrating network pharmacology,in vitro experimental validation,and chemometric analysis,rosmarinic acid,neochlorogenic acid,caffeic acid,salvigenin,luteolin,ferulic acid,quercetin-3-O-β-D-glucuronide,acacetin,salvianolic acid B and chlorogenic acid may be the Q-Markers of H.cuspidatus against asthma.关键词
硬尖神香草/质量标志物/支气管哮喘/气道重塑/网络药理学/指纹图谱Key words
Hyssopus cuspidatus/quality markers/bronchial asthma/airway remodeling/network pharmacology/fingerprint分类
医药卫生引用本文复制引用
蔡晓翠,郭君婷,赵婷婷,刘桂花..硬尖神香草抗支气管哮喘气道重塑的质量标志物研究[J].中国药房,2026,37(6):733-739,7.基金项目
中央医疗机构中药质量提升项目(No.2025) （No.2025）
新疆维吾尔自治区公益性科研院所基本科研业务经费资助项目(No.KY2024127) （No.KY2024127）
新疆维吾尔自治区自然科学基金面上项目(No.2022D01A132) （No.2022D01A132）
