广东海洋大学学报2026，Vol.46Issue(1)：45-55,11.DOI:10.3969/j.issn.1673-9159.2026.01.005
不同年龄高体鰤精巢转录组分析
Comparative Transcriptome Analysis of Testis Development across Different Ages in Seriola dumerili
摘要
Abstract
[Objective]This study aims to investigate the molecular mechanisms regulating testicular development in greater amberjack(Seriola dumerili)at different age stages under artificial aquaculture conditions.[Methods]Histological observation,transcriptome sequencing analysis,gene functional annotation,and screening and identification of differentially expressed genes(DEGs)were performed on the testis of 1-year-old and 2-year-old greater amberjack.The expression levels of genes related to testicular development were detected using real-time quantitative PCR(qPCR).[Results]Histological analysis showed that the testis of 1-year-old fish were at Stage I,while those of 2-year-old fish were at Stage IV.Transcriptome sequencing of testis from 1 and 2 age groups yielded 263 476 570 clean reads,corresponding to 21 258 annotated genes,among which 1 108 were identified as differentially expressed genes(DEGs).Functional enrichment analysis revealed that numerous DEGs were involved in reproduction-related metabolic pathways,including steroid hormone biosynthesis,GnRH signaling pathway,TGF-beta signaling pathway,and Wnt signaling pathway.Both transcriptome data and qPCR results indicated that,compared with 1-year-old fish,11 genes,namely spata18,spata6,sycp3,smc3,sycp2,mns1,spag17,rsph1,spef2,spa17,and spaca6 were significantly up-regulated in the testis of 2-year-old fish,while 5 genes,namely gsdf,gdf3,and foxl2,cyp19a1a,and bmp8 were significantly down-regulated.[Conclusion]Through transcriptome sequencing analysis of testis from 1-year-old and 2-year-old greater amberjack,this study identified age-related differentially expressed genes and meta-bolic pathways involved in testicular development,preliminarily revealing regulation mechanisms of testis development in S.dumerili at the molecular level.关键词
高体鰤/不同年龄/精巢/转录组Key words
Seriola dumerili/different ages/testis/transcriptome分类
农业科技引用本文复制引用
林浩毅,邓秋霞,黄洋,茹笑影,杨佳慧,李广丽,朱春华..不同年龄高体鰤精巢转录组分析[J].广东海洋大学学报,2026,46(1):45-55,11.基金项目
广东海洋大学自科项目(030301022301) （030301022301）
湛江湾实验室人才团队引进项目(ZJW-2023-10) （ZJW-2023-10）
广东省现代化海洋牧场适养品种核心技术攻关项目(2024-MRB-00-001) （2024-MRB-00-001）
