广东海洋大学学报2026，Vol.46Issue(1)：56-62,7.DOI:10.3969/j.issn.1673-9159.2026.01.006
不同生长速率高体鰤消化、抗氧化及免疫性能差异
Differences in Digestive,Antioxidant,and Immune Performance in Seriola dumerili with Different Growth Rates
摘要
Abstract
[Objective]To investigate the digestive,antioxidant,and immune performance of greater amberjack(Seriola dumerili)with different growth rates under the same aquaculture environment,and explore the reasons behind the differences in growth rates.[Method]The fish that were raised to one year old under identical feeding conditions were categorized into a fast-growth group and a slow-growth group.Ten fish were randomly selected for each group to measure their total length,body mass,and body height.Additionally,the activities of digestive enzymes in the liver,stomach,and intestine were assessed,along with the activities of antioxidant enzymes and non-specific immune enzymes in serum and liver.Furthermore,the expression of immune-and antioxidant-related genes in the liver were analyzed.[Result]The results of the digestive enzyme assays indicated that the activities of pepsin(PPS),lipase(LPS),and trypsin(TPS)in the liver and of amylase(AMS)and TPS in the intestine and stomach,were significantly elevated compared to those in the fast-growing group(P<0.05).In contrast,PPS activity was significantly lower than that in the slow-growing group(P<0.05).The antioxidant enzyme assay results revealed that the activity of catalase(CAT)and the content of malondialdehyde(MDA)in the liver,as well as the glutathione peroxidase(GPX),CAT,and superoxide dismutase(SOD)in the serum,were significantly higher in the fast-growing group than those in the slow-growing group.Conversely,SOD activity in the liver and MDA content in the serum were significantly lower in the fast-growing group than those in the slow-growing group(P<0.05).Non-specific enzyme activity assay results showed that aspartate aminotransferase(AST)and alanine aminotransferase(ALT)activities in the liver,as well as alkaline phosphatase(AKP)and acid phosphatase(ACP)activities in the serum,were significantly higher in the fast-growing group than those in the slow-growing group(P<0.05).However,AST and ALT activities in the serum were significantly lower than those in the slow-growing group(P<0.05).The qPCR results indicated that the expression levels of the antioxidant genes gpx1b,cat,sod1,sod3,and gss,as well as the immune-related genes il10 and tgfβ2,were significantly higher in the fast-growing group(P<0.05).[Conclusion]The fast-growing greater amberjack exhibits enhanced digestive,antioxidant,and immune capabilities,providing a theoretical basis for exploring the reasons behind differences in growth rates among individuals of this species.关键词
高体鰤/生长/消化酶/抗氧化酶/非特异性免疫酶/基因表达Key words
Seriola dumerili/growth/digestive enzymes/antioxidant enzymes/non-specific immune enzymes/gene expression分类
农业科技引用本文复制引用
李晓净,茹笑影,黄洋,邓秋霞,陈培培,郭玉婷,林浩毅,朱春华..不同生长速率高体鰤消化、抗氧化及免疫性能差异[J].广东海洋大学学报,2026,46(1):56-62,7.基金项目
广东海洋大学自科项目(030301022301) （030301022301）
湛江湾实验室人才团队引进项目(ZJW-2023-10) （ZJW-2023-10）
广东省现代化海洋牧场适养品种核心技术攻关项目(2024-MRB-00-001) （2024-MRB-00-001）
2024年湛江市科技计划项目(2024R1010) （2024R1010）
2025年湛江市科技计划项目(2025R02113) （2025R02113）
广东省"扬帆计划"-2025年现代化海洋牧场产业人才振兴计划子项目(HYJ-YFJH-2025001) （HYJ-YFJH-2025001）
