浙江农业学报2026，Vol.38Issue(2)：269-283,15.DOI:10.3969/j.issn.1004-1524.20241050
膜荚黄芪TCP基因家族全基因组分析及其对非生物胁迫的响应
Genome-wide analysis of the TCP gene family in Astragalus membranaceus(Fisch.)Bunge and its response to abiotic stress
摘要
Abstract
This study aimed to identify and characterize the TCP gene family in Astragalus membranaceus(Fisch.)Bunge and elucidate its role in abiotic stress responses,and provide a theoretical basis for investigating the functional role of TCP genes in Astragalus membranaceus(Fisch.)Bunge under abiotic stress conditions.A systematic analysis of the TCP genes in Astragalus membranaceus(Fisch.)Bunge and their encoded amino acid sequences was conduc-ted using bioinformatics methods.RNA-seq technology was employed to investigate the expression patterns of TCP genes in different tissues(roots,stems,and leaves)of Astragalus membranaceus(Fisch.)Bunge.Additionally,the expression profiles of five representative AmTCP genes under abiotic stress conditions(high temperature,drought,and salt stress)were validated through real-time quantitative PCR(RT-qPCR).The results showed that a total of 24 TCP genes were identified and unevenly distributed on 8 chromosomes of Astragalus membranaceus(Fisch.)Bunge.The 24 AmTCP genes can be classified into Class Ⅰ(PCF)and Class Ⅱ(CYC/TB1 and CIN).Proteins within the same subfamily had highly similar motif compositions,and most AmTCP genes lack introns.Fragment duplication played an important role in the evolution of the TCP gene family in Astragalus membranaceus(Fisch.)Bunge.In ad-dition,analysis of the promoter region revealed multiple light-responsive elements and hormone-responsive elements.Tissue expression analysis showed that the AmTCP genes had significant tissue expression specificity.Under abiotic stress,there were significant differences in the expression levels of five representative AmTCP genes,among which AmTCP18 and AmTCP19 genes showed higher expression levels under various stress conditions.In summary,AmTCP18 and AmTCP19 may play a key role in the response of Astragalus membranaceus(Fisch.)Bunge to abiotic stress.This study lays the foundation for further functional and evolutionary research on the TCP gene family of As-tragalus membranaceus(Fisch.)Bunge.关键词
膜荚黄芪/TCP基因家族/全基因组分析/非生物胁迫Key words
Astragalus membranaceus(Fisch.)Bunge/TCP gene family/genome-wide analysis/abiotic stress分类
农业科技引用本文复制引用
马琦洋,黄雪莲..膜荚黄芪TCP基因家族全基因组分析及其对非生物胁迫的响应[J].浙江农业学报,2026,38(2):269-283,15.基金项目
省级创业训练计划(S202310344003X) （S202310344003X）
2023 浙江省新苗人才计划(2023R410004) （2023R410004）
