浙江中医杂志2026，Vol.61Issue(3)：189-194,6.
灯盏花素对急性肺栓塞的血管内皮保护作用及抗炎机制研究
Study on the Protective Effect of Breviscapine on Vascular Endothelium and Its Anti-inflammatory Mechanism in Acute Pulmonary Embolism
摘要
Abstract
Objective:To investigate the protective effect and anti-inflammatory mechanism of breviscapine(Bre)on vascular endothelium in acute pulmonary embolism(APE).Methods:SD rats were randomly divided into Sham group,APE group,APE+low molecular weight heparin calcium injection(APE+LMWH)group,APE+breviscapine low-dose(APE+Bre-L)group,and APE+breviscapine high-dose(APE+Bre-H)group.APE rat models were constructed using autologous thrombus.Mean pulmonary artery pressure(mPAP)and right ventricular pressure(RVP)were measured using an electrocardiogram monitor,and arterial oxygen partial pressure(PaO2)and arterial carbon dioxide partial pressure(PaCO2)were measured.Lung tissue pathologi-cal damage was examined by HE staining.Human pulmonary artery endothelial cells(HPAECs)were stimu-lated with thrombin to construct an in vitro cell model,followed by intervention with Bre and TLR4 agonist LPS.Cell viability,serum dimer(DD),interleukin-1 β(IL-1 β),tumor necrosis factor-α(TNF-α),monocyte chemoattractant protein-1(MCP-1),endothelin-1(ET-1),thromboxane B2(TXB2)levels,and von Willebrand factor(vWF),vascular endothelial cell adhesion molecule-1(VCAM-1),and P-selectin levels in HPAECs were assessed using CCK8 and ELISA.Nuclear translocation of NF-κ B p65 in HPAECs was determined by immunofluorescence assay.The expression of TLR-4/MyD88/NF-κB pathway proteins was de-termined by Western blot.Results:Compared with the Sham group,APE rats showed severe pathological damage,with increased mPAP,RVP,DD,TNF-α,IL-1 β,MCP-1,ET-1,TXB2,TLR-4,MyD88,and p-NF-κ B/NF-κ B expression,while decreased PaO2 levels.LMWH and Bre-H treatments in APE rats improved the above-mentioned indicators.In HPAECs stimulated by thrombin,Bre increased cell viability,reduced vWF,VCAM-1,and P-selectin levels,inhibited nuclear translocation of NF-κ B p65,and down-regulated TLR-4,MyD88,and p-NF-κ B/NF-κ B expression.In addition,LPS weakened the above ef-fects.Conclusion:Bre may alleviate the inflammatory response and endothelial dysfunction to improve APE,which may be closely related to the inhibition of TLR-4/MyD88/NF-κ B pathway.关键词
灯盏花素/急性肺栓塞/炎症反应/内皮功能障碍/TLR-4/MyD88/NF-κB通路Key words
breviscapine/acute pulmonary embolism/inflammatory response/endothelial dysfunction/TLR-4/MyD88/NF-κ B pathway引用本文复制引用
张言涛,吴丽丽,周黎明..灯盏花素对急性肺栓塞的血管内皮保护作用及抗炎机制研究[J].浙江中医杂志,2026,61(3):189-194,6.基金项目
浙江省中医药科技计划项目(2024ZL661) （2024ZL661）
