植物遗传资源学报2026，Vol.27Issue(3)：506-515,10.DOI:10.13430/j.cnki.jpgr.20251106001
菜心Brassica oxyrrhina胞质不育系的转育研究
Development of a Cytoplasmic Male-Sterile Line in Flowering Chinese Cabbage(Brassica rapa ssp.parachinensis)through Alloplasmic Substitution with Brassica oxyrrhina Cytoplasm
摘要
Abstract
Using a cytoplasmic male sterile(CMS)line of Brassica napus carrying cytoplasm from Brassica oxyrrhina as the maternal parent,we developed a stable CMS line in flowering Chinese cabbage(Brassica rapa subsp.parachinensis)through interspecific hybridization followed by six backcrosses,assisted by embryo rescue and a speed breeding system.The line was comprehensively characterized for morphological traits,pollen fertility,seed-setting performance,and cytoplasm-specific molecular markers.The CMS line exhibited typical floral morphology of flowering Chinese cabbage,featuring degenerated stamens with shortened filaments and shriveled anthers while nectaries developed normally.Acetic acid-carmine staining revealed aborted pollen grains,characterized by small,round,anucleate,and stained pale brown.Scanning electron microscopy further showed a lack of sporopollenin deposition and the collapse of germination apertures in the sterile pollen,consistent with classical CMS phenotypes.Field evaluations confirmed complete self-sterility;however,under open pollination,plants produced an average of 12 seeds per silique,indicating strong outcrossing compatibility.Comparative mitochondrial genome analysis enabled the development of a cytoplasm-specific KASP marker,KOX3,which reliably discriminates cytoplasm from Brassica oxyrrhina(C/C genotype)from other cytoplasmic types(T/T genotype).This novel CMS line provides a valuable germplasm resource for heterosis breeding in flowering Chinese cabbage,and the KOX3 marker offers a robust molecular tool for rapid cytoplasm identification and purity assessment of CMS lines.关键词
菜心/雄性不育/异交结实/KASP标记Key words
flowering Chinese cabbage/male sterility/outcrossing seed set/KASP marker引用本文复制引用
吴增祥,杨易,黎庭耀,沈卓,周轩,钟妍婷,张艳..菜心Brassica oxyrrhina胞质不育系的转育研究[J].植物遗传资源学报,2026,27(3):506-515,10.基金项目
广东省乡村振兴战略专项种业振兴项目(2024-NPY-00-006,2024-NJS-00-007) （2024-NPY-00-006,2024-NJS-00-007）
广州市科学技术局2024年度基础与应用基础研究专题(青年博士"启航"项目)(2024A04J3499)Guangdong Provincial Special Program for Rural Revitalization Strategy-Seed Industry Revitalization Projects(2024-NPY-00-006,2024-NJS-00-007) （青年博士"启航"项目）
Guangzhou Science and Technology Bureau's 2024 Basic and Applied Basic Research Program(Youth PhD"Qihang"Initiative)(2024A04J3499) （Youth PhD"Qihang"Initiative）
