中药新药与临床药理2026，Vol.37Issue(3)：437-446,10.DOI:10.19378/j.issn.1003-9783.2026.03.006
山仙颗粒通过调节肠道菌群-炎症反应轴抑制肝癌前病变的机制
Mechanistic Study on Shanxian Granules Inhibiting Hepatic Precancerous Lesions by Modulating the Gut Microbiota-Inflammatory Response Axis
摘要
Abstract
Objective To investigate the mechanism by which Shanxian Granules delay the progression of hepatic precancerous lesions via regulating gut microbiota composition and suppressing inflammatory factors.Methods Eighty SD rats were randomly divided into a normal group,a model group,and low-and high-dose Shanxian Granules groups.A rat model of hepatic precancerous lesions was established by intraperitoneal injection of diethylnitrosamine(DEN).Intragastric administration began on the second day of modeling,once daily for 16 consecutive weeks.Blood biochemical analysis was performed to assess liver function indices.Morphological changes in liver and intestinal tissues were observed by HE staining.Changes in gut microbiota were detected using 16S rRNA high-throughput sequencing.Immunohistochemistry was used to detect the proliferation cell-related antigen Ki67.Western Blot was employed to measure the expression of inflammation-related proteins.Results Compared with the normal group,the model group showed increased levels of liver function-related enzymes(P＜0.05),significant histopathological changes in the liver,enhanced expression of Ki67 in hepatocytes(P＜0.05),a decrease in beneficial bacteria(Firmicutes,Lactobacillus)(P＜0.05),an increase in pathogenic bacteria(Proteobacteria,Bacteroidetes)(P＜0.05),progressive damage to the small intestinal mucosa,and elevated expression of inflammation-related proteins.Compared with the model group,the low-and high-dose Shanxian Granules groups exhibited decreased levels of liver function-related enzymes(P＜0.05,P＜0.01),improved liver pathological damage,reduced expression of Ki67 in hepatocytes(P＜0.05,P＜0.01),an increase in beneficial bacteria(P＜0.05),a decrease in pathogenic bacteria(P＜0.05),recovery of small intestinal mucosal damage,and decreased expression of inflammation-related proteins(P＜0.05).Conclusion Shanxian Granules can ameliorate DEN-induced hepatic precancerous lesions in rats by improving gut microbiota dysbiosis,repairing intestinal mucosal damage,and reducing the expression of inflammation-related proteins in liver tissue.关键词
山仙颗粒/肠道菌群/炎症/肝癌前病变/炎癌转化/大鼠Key words
Shanxian Granules/gut microbiota/inflammation/hepatic precancerous lesions/inflammation-cancer transformation/rats分类
医药卫生引用本文复制引用
梁玉,谢敏,王婕,杨鑫茂,李宏,王院春,潘艳芳..山仙颗粒通过调节肠道菌群-炎症反应轴抑制肝癌前病变的机制[J].中药新药与临床药理,2026,37(3):437-446,10.基金项目
国家自然科学基金项目(81703842) （81703842）
陕西省重点研发计划项目(2024SF-YBXM-147) （2024SF-YBXM-147）
秦创原中医药产业创新聚集区项目(L2024-QCY-ZYYJJQ-X217) （L2024-QCY-ZYYJJQ-X217）
陕西中医药大学研究生创新实践能力提升项目(CXSJ202503) （CXSJ202503）
咸阳市重点研发计划项目(L2025-ZDYF-JBFZ-017). （L2025-ZDYF-JBFZ-017）
