中医药学报2026，Vol.54Issue(3)：6-14,9.DOI:10.19664/j.cnki.1002-2392.260048
养血安胎方含药血清调节外泌体释放影响内皮细胞及子宫内膜细胞的机制研究
Study on the Mechanism of Medicated Serum from Yangxue Antai Formula in Influencing Endothelial and Endometrial Cells via Regulating Exosome Release
摘要
Abstract
Objective:To investigate the mechanism of Yangxue Antai serum in regulating the release of exosomes from JEG-3 cells to affect endothelial cells and endometrial cells.Methods:The exosome release inhibitor GW4869 was used to inhibit the release of exosomes from JEG-3 cells,and the Yangxue Antai serum interfered with JEG-3 cells,A co-culture system was established in which JEG-3 cells or exosomes secreted by JEG-3 cells were co-cultured with HEC-1-A cells or HUVECs.The expression of VEGFA and p-VEGFR2 in HUVECs,the expression of EMT-related proteins E-cadherin,Vimentin,N-cadherin and Fibronectin as well as the expression of deciduation-related proteins BMP2,HOXA10,PRL and IGFBP1 in HEC-1-A cells were measured by Western blot.The migration rate of HUVECs was measured by cell scratch assay.The invasion and migration ability of HUVECs were measured by Transwell assay,F-actin expression was measured by cytoskeleton assay,and the lumen length in HUVECs was measured by tubule formation assay.Results:Compared with Control group,the expressions of VEGFA and p-VEGFR2 in GW4869 group were significantly decreased.The expression of E-cadherin was significantly increased,while the expression of Vimentin,N-cadherin and Fibronectin were significantly decreased.The expressions of BMP2,HOXA10,PRL and IGFBP1 were significantly decreased.Cell invasion and migration ability were significantly decreased,the lumen length was decreased,and the expression of F-actin was significantly decreased.Compared with GW4869 group,the expressions of VEGFA and p-VEGFR2 in GW4869+Yangxue Antai and GW4869+exosome group were significantly increased.The expression of E-cadherin was significantly decreased,while the expression of Vimentin,N-cadherin and Fibronectin was significantly increased.The expressions of BMP2,HOXA10,PRL and IGFBP1 were significantly increased.Cell invasion and migration ability were increased significantly,the lumen length was increased,and the expression of F-actin was increased significantly.Conclusion:Yangxue Antai serum can affect the angiogenesis of endothelial cells,EMT and decidualization of endometrial cells by regulating the release of exosomes from JEG-3 cells.关键词
外泌体/血管生成/上皮细胞-间质转化/蜕膜化Key words
Exosome/Angiogenesis/Epithelial-mesenchymal transformation/Decidualization分类
医药卫生引用本文复制引用
姚伟洁,冯欣..养血安胎方含药血清调节外泌体释放影响内皮细胞及子宫内膜细胞的机制研究[J].中医药学报,2026,54(3):6-14,9.基金项目
国家自然科学基金项目(82204783) （82204783）
