北方园艺Issue(6)：10-17,8.DOI:10.11937/bfyy.20253405
菊花CmbZIP53基因克隆与表达分析
Cloning and Expression Analysis of the CmbZIP53 Gene in Chrysanthemum morifolium
摘要
Abstract
Taking the Chrysanthemum morifolium cultivar'Jinba'as experimental material,the CmbZIP53 gene and its promoter sequence were cloned based on the transcriptomic data of C.morifolium under waterlogging stress.Bioinformatic analysis and waterlogging expression analysis were conducted to explore the potential role of CmbZIP53 in the response to waterlogging stress,in order to provide reference for further research on the function of bZIP transcription factors and to identify key genes related to waterlogging tolerance in C.morifolium.The results showed that the length of open reading frame(ORF)of CmbZIP53 was 417 bp,encoding 138 amino acids.Protein structure prediction revealed that CmbZIP53 contained a typical BRLZ domain of bZIP proteins,indicating its belonging to the bZIP protein family,with the closest relationship to the CibZIP29 protein of Chrysanthemum indicum.Waterlogging stress significantly induced the expression of CmbZIP53,and its induction level progressively increased with prolonged waterlogging duration.The promoter sequence was found to contain multiple cis-acting elements,including anaerobic induction-responsive elements and hormone signaling-responsive elements,suggesting that CmbZIP53 may be involved in the waterlogging response process in C.morifolium.关键词
菊花/CmbZIP53/基因克隆/表达模式Key words
Chrysanthemum morifolium/CmbZIP53/gene cloning/expression pattern分类
农业科技引用本文复制引用
牛少君,杨帆,洪煌楷,袁美佳,赵楠..菊花CmbZIP53基因克隆与表达分析[J].北方园艺,2026,(6):10-17,8.基金项目
国家自然科学基金资助项目(32401659) （32401659）
福建省自然科学基金资助项目(2022J01907) （2022J01907）
闽南师范大学校长基金资助项目(KJ2020005). （KJ2020005）
