北方园艺Issue(6)：27-32,6.DOI:10.11937/bfyy.20253384
两个枸杞品种不同器官褪黑素含量及其合成酶基因表达
Melatonin Content and Expression of Biosynthetic Gene in Different Organs of Two Lycium barbarum L.Cultivars
摘要
Abstract
Taking the fresh roots,stems,leaves,and flowers of'Ningqi 1'and'Ningqicai 1'sampled in summer as the experimental materials,high-performance liquid chromatography(HPLC)and quantitative real-time polymerase chain reaction(qRT-PCR)were utilized to investigate melatonin contents and the expression profiles of melatonin biosynthetic genes across different organs,in order to provide reference for the subse quent research on the synthesis of melationin in Lycium barbarum L.and the exploration of its medicinal value.The results showed that the melatonin content in each organ of'Ningqicai 1'was significantly higher than that of'Ningqi 1'.For both cultivars,the melatonin content followed the descending order of flower＞leaf＞stem＞root.Additionally,the four biosynthetic genes,namely TDC,T5H,COMT00,and COMT01,were ubiquitously expressed in roots,stems,leaves,and flowers.关键词
枸杞/褪黑素含量/基因表达/褪黑素合成酶Key words
Lycium barbarum L./melatonin content/gene expression/melatonin biosynthetic enzymes分类
农业科技引用本文复制引用
周梓晗,杨建帮,沙凤霞,姚文孔,张宁,冯美..两个枸杞品种不同器官褪黑素含量及其合成酶基因表达[J].北方园艺,2026,(6):27-32,6.基金项目
宁夏自然科学基金资助项目(2024AAC02026) （2024AAC02026）
国家自然科学基金资助项目(3236140164). （3236140164）
