北方园艺Issue(6)：33-39,7.DOI:10.11937/bfyy.20253079
基于转录组分析除草剂对羊肚菌的毒理机制
Elucidation of the Toxicological Mechanism of Herbicides in Morchella sextelata Based on Transcriptomic Analysis
摘要
Abstract
Taking the Morchella sextelata strain M6611 as the test material,the PDA plate culture method was adopted and combined with RNA-seq transcriptome analysis to study the effects of four commonly used herbicides(trifluralin,pendimethalin,S-metolachlor and acetochlor))on the mycelial growth of the mushroom,in order to provide references for analyzing the toxicological mechanism of herbicides on morel mushrooms.The results showed that all herbicides suppressed mycelial growth,with S-metolachlor exhibiting relatively weaker inhibition.Comprehensive transcriptome analysis revealed that herbicide treatment severely impaired critical energy metabolism pathways,particularly the processes of glycolysis/gluconeogenesis and the glyoxylate cycle.In conclusion,herbicides should be used cautiously in M.sextelata cultivation,and soil herbicide residue monitoring should be enhanced to reduce potential risks of mycelial growth inhibition and yield loss.关键词
'六妹'羊肚菌/除草剂/转录组学分析/毒理学Key words
Morchella sextelata/herbicides/transcriptomic analysis/toxicology分类
农业科技引用本文复制引用
党永祥,蒋长生,杨琴,张晋铭,朱学泰..基于转录组分析除草剂对羊肚菌的毒理机制[J].北方园艺,2026,(6):33-39,7.基金项目
国家自然科学基金资助项目(31770588) （31770588）
2024年度兰州市第八批科技计划资助项目(2024-8-26). （2024-8-26）
