发光学报2026，Vol.47Issue(3)：389-399,11.DOI:10.37188/CJL.20250278
水相稳定钙钛矿量子点生物探针制备及其在胱抑素C免疫层析中的应用
Preparation of Water-stable Perovskite Quantum Dot Bioprobes and Their Application in Cystatin C Lateral Flow Immunoassay
摘要
Abstract
Cystatin C(Cys C)is an important biomarker for evaluating and monitoring renal function and is widely used for early screening of kidney disease.Fluorescent lateral flow immunoassay is a convenient approach for rapid and quantitative Cys C testing,yet its analytical performance is often constrained by the brightness and stability of conven-tional signal labels.Here,we report a water-stable perovskite quantum dot(PQD)-based fluorescent bioprobe for use in Cys C LFIA.Mesoporous silica nanoparticles(MSNs)were employed as nanocarriers,enabling in situ formation of CsPbBr3 quantum dots within the mesopores while a SiO2 sealing layer was simultaneously constructed.The resulting CsPbBr3@MSNs@SiO2 probes exhibited high photoluminescence quantum yield(PLQY≈93%)and excellent water stability(>120 h),providing robust signal output for LFIA readout.Based on this bioprobe,a fluorescent LFIA strip was fabricated for quantitative Cys C detection,demonstrating good specificity and a clear concentration-dependent response.This work offers a bright and water-stable labeling strategy for point-of-care testing of renal function-related biomarkers and expands the utility of metal halide PQDs in bioanalysis and LFIA-based diagnostics.关键词
钙钛矿量子点/胱抑素C/免疫层析/荧光/即时检测Key words
perovskite quantum dots/Cystatin C/lateral flow immunoassay/fluorescence/point-of-care testing分类
数理科学引用本文复制引用
陈志山,朱宏强,毛梓晴,林紫依,张绍安,徐佳,李战军,李杨..水相稳定钙钛矿量子点生物探针制备及其在胱抑素C免疫层析中的应用[J].发光学报,2026,47(3):389-399,11.基金项目
国家自然科学基金(52572173,52402192) （52572173,52402192）
广州市重点研发计划(2023B03J1239) （2023B03J1239）
宁波大学高级科学研究项目(GJPY2025010)Supported by National Natural Science Foundation of China(52572173,52402192) （GJPY2025010）
Guangzhou Key Research and Develop-ment Program(2023B03J1239) （2023B03J1239）
High-level Scientific Research Project of Ningbo University(GJPY2025010) （GJPY2025010）
