西部中医药2026，Vol.39Issue(3)：28-32,5.DOI:10.12174/j.issn.2096-9600.2026.03.06
罗汉果玉竹颗粒对脂多糖诱导大鼠急性肺损伤中Fibronectin、Collagen I及α-SMA表达的影响
Effects of Luohanguo Yuzhu Granules on the Expressions of Fibronectin,Collagen I and α-SMA in Rats with LPS-induced ALI
摘要
Abstract
Objective:To study the protective effects of Luohanguo(Siraitiae fructus)Yuzhu(Polygonati odorati rhizoma)granules on LPS-induced ALI in rats and its potential mechanism.Methods:All 58 SPF-grade Wistar rats were randomized into the blank control group(K group,10 rats),model control group(M group,12 rats),low-dose Luohanguo Yuzhu granules group(LY-L group,3.24 g·kg-1,12 rats),high-dose Luohanguo Yuzhu granules group(LY-H group,6.48 g·kg-1,12 rats)and prednisone positive control group(PN group,5.4 mg·kg-1,12 rats)according to body weight.Except for K group,the rats in the remaining groups received LPS solution(6 mg·kg-1)via tail vein injection.After successfully modeling,the treatment groups received the corresponding drugs by gavage once daily,administration volume was 10mL·kg-1;K group and M group were given the same volume of distilled water.After seven consecutive days of administration,the rats were anesthetized with an intraperitoneal injection of 10％chloral hydrate(3.5 mL·kg-1),and blood samples were collected from the abdominal aorta,ELISA was adopted to detect the contents of IL-6;lung tissues were collected for hematoxylin-eosin(HE)staining to observe pathological changes.Immunohistochemistry(IHC)was adopted to measure the expressions of FN,COL1A1 and α-SMA.Results:Compared with K group,M group observed disrupted alveolar structure,markedly thickened alveolar septa,and extensive congestion,edema,and inflammatory cell infiltration in the alveolar walls;an increase in the contents of IL-6(P<0.01),the elevated expressions of FN,COL1A1 and α-SMA in lung tissue(P<0.01).Compared with M group,LY-L group and LY-H group demonstrated a decrease in the contents of IL-6(P<0.01),a significant improvement in lung histopathological injury,the reduction in the expressions of FN,COL1A1 and α-SMA(P<0.01).Pearson correlation analysis showed that the contents of IL-6 were positively related to the expressions of FN,COL1A1 and α-SMA in lung tissue(P<0.01).Conclusion:Luohanguo Yuzhu granules have a protective effect against LPS-induced ALI in rats,and its mechanism might be attributed to the attenuation of the inflammatory response in lung tissue,the inhibition of IL-6 release,and the subsequent downregulation of FN,COL1A1,and α-SMA expression in the lungs.关键词
急性肺损伤/罗汉果玉竹颗粒/脂多糖/纤连蛋白/I型胶原/α平滑肌肌动蛋白Key words
acute lung injury/Luohanguo Yuzhu granules/lipopolysaccharide/fibronectin/collagen I/α-smooth muscle actin分类
医药卫生引用本文复制引用
赵丽华,康晓娜,韩小江..罗汉果玉竹颗粒对脂多糖诱导大鼠急性肺损伤中Fibronectin、Collagen I及α-SMA表达的影响[J].西部中医药,2026,39(3):28-32,5.基金项目
内蒙古自治区自然科学基金项目[2020MS(LH)0812]. （LH）
