华中农业大学学报2026，Vol.45Issue(2)：28-36,9.DOI:10.13300/j.cnki.hnlkxb.2026.02.004
前臀鮡和贡山鮡精子的超微结构与运动学参数比较
Comparative study on sperm ultrastructure and kinematic parameters in Pareuchiloglanis anteanalis and Pareuchiloglanis gongshanensis
摘要
Abstract
To investigate the sperm ultrastructure and kinematic characteristics of Pareuchiloglanis an-teanalis and Pareuchiloglanis gongshanensis,a systematic comparative analysis was conducted using scan-ning electron microscopy(SEM),transmission electron microscopy(TEM),and a computer-assisted sperm analysis(CASA)system.The results revealed that,although the spermatozoa of both species con-sist of an acrosome-less head,a midpiece,and a single flagellum,they exhibit significant divergences in morphological configuration and swimming patterns.The spermatozoa of P.anteanalis were relatively lon-ger,characterized by a slender head(length-to-width ratio:1.51),a midpiece containing four umbrella-shaped,tightly arranged mitochondria,and a flagellum with symmetrical lateral fins.In contrast,the sper-matozoa of P.gongshanensis were shorter,featuring a nearly spherical head(ratio:1.08),a midpiece with 4-5 smaller mitochondria,and a shorter flagellum with more developed lateral fins.CASA measurements revealed no significant differences in average path velocity(VAP),straight-line velocity(VSL),or curvi-linear velocity(VCL)between the two species.However,P.anteanalis sperm exhibited significantly high-er straightness(STR)and beat-cross frequency(BCF)(P<0.05),resulting in a more linear swimming trajectory.Conversely,P.gongshanensis sperm showed a significantly greater amplitude of lateral head dis-placement(ALH)(P<0.05)and a more curved movement path.This study indicates that P.anteanalis sperm exhibit the classic"introsperm"phenotype adapted for internal fertilization,whereas P.gongshanen-sis sperm display traits characteristic of"aquasperm",reflecting an adaptation to external fertilization.关键词
前臀鮡/贡山鮡/精子超微结构/运动学参数/授精方式Key words
Pareuchiloglanis anteanalis/Pareuchiloglanis gongshanensis/sperm ultrastructure/kine-matic parameters/insemination strategy分类
农业科技引用本文复制引用
汪灏,熊皓,金阳,范雅文,杨学芬,赵恒,杨瑞斌..前臀鮡和贡山鮡精子的超微结构与运动学参数比较[J].华中农业大学学报,2026,45(2):28-36,9.基金项目
华电集团金沙江上游远期放流鱼种人工繁育技术研究项目(T-2022-04) （T-2022-04）
