华中农业大学学报2026，Vol.45Issue(2)：80-86,7.DOI:10.13300/j.cnki.hnlkxb.2026.02.009
木薯普通花叶病毒CsCP与MeGRXC3互作分析
Interaction between Cassava common mosaic virus CsCP and MeGRXC3 protein
摘要
Abstract
Cassava common mosaic virus(CsCMV)is the primary agent causing cassava mosaic dis-ease.Its capsid protein(CP)plays a crucial role in virus-plant interactions during infection.Glutaredoxins(GRXs)are thiol oxidoreductases with low molecular weight,among which CC-type GRXs are key regu-lators of resistance to plant disease.Given that CP is a key pathogenicity factor of CsCMV involved in mod-ulating host immune responses,this article investigated whether CsCP interacts with MeGRXC3 to syner-gistically regulate redox homeostasis and thereby modulate viral infection.The yeast two-hybrid(Y2H),bi-molecular fluorescence complementation(BiFC),and subcellular localization assays were used to examine the interaction between CsCP and MeGRXC3.The results showed that there was an interaction between cassava MeGRXC3 and CsCP in yeast cells.Green fluorescence appeared at 488 nm after co-expressing Me-GRXC3 and CsCP in Nicotiana benthamiana epidermal cells,confirming the interaction between CsCP and MeGRXC3 in plant cells.CsCP and MeGRXC3 exhibited co-localization in Nicotiana benthamiana epi-dermal cells.It is indicated that there is an interaction between CsCP and MeGRXC3.It will lay the ground-work for further elucidating the molecular mechanisms by which CP facilitates the infection of CsCMV.关键词
木薯普通花叶病毒/木薯普通花叶病毒外壳蛋白(CsCP)/木薯谷氧还蛋白(MeGRXC3)/蛋白互作/酵母双杂交/双分子荧光互补Key words
Cassava common mosaic virus/CsCP/MeGRXC3/protein interactions/Y2H/BiFC分类
农业科技引用本文复制引用
白双琴,谢秋贤,刘雪婷,符艳,叶彤,阮孟斌,张秀春,任艳利..木薯普通花叶病毒CsCP与MeGRXC3互作分析[J].华中农业大学学报,2026,45(2):80-86,7.基金项目
国家自然科学基金项目(32560526) （32560526）
热带作物生物育种全国重点实验室项目(NKLTCBCXTD29) （NKLTCBCXTD29）
中国热带农业科学院国家热带农业科学中心科技创新团队项目(CATASCXTD202301) （CATASCXTD202301）
中央公益性科研院所基本科研业务费专项(1630032025010) （1630032025010）
