华中农业大学学报2026，Vol.45Issue(2)：99-112,14.DOI:10.13300/j.cnki.hnlkxb.2026.02.011
莴苣全基因组InDel标记开发及应用
Development and application of genome-wide InDel markers in lettuce
摘要
Abstract
The genome sequences of Salinas(lettuce)and YL1(stem lettuce)were compared to elu-cidate the genetic differences among the main cultivated types of lettuce(Lactuca sativa)including crisp-head lettuce,Youmaicai,and stem lettuce and resolve the issue of synonymy.23 901 potential InDel loci were identified across the whole genome.80 loci were randomly selected for designing primers.42 stably polymorphic markers were obtained from screening with PCR across six accessions of stem lettuce and six accessions of lettuce.57 commercial varieties including 10 crisphead lettuce,24 stem lettuce,and 23 You-maicai were subsequently genotyped with polymorphic markers obtained.The results showed that the num-ber of markers suitable for distinguishing crisphead lettuce,stem lettuce,and Youmaicai was 14,17,and 35 out of the 42 polymorphic markers,respectively.The 10 varieties of crisphead lettuce were classified into 8 groups,the 24 varieties of stem lettuce into 20 groups,and the 23 varieties of Youmaicai into 12 groups,meaning that there are genetic differences and synonymy among varieties.It is indicated that the developed InDel marker system can be directly used for the rapid identification and clarification of genetic background in varieties of lettuce.关键词
莴苣/InDel标记/多态性/品种鉴定/聚类分析Key words
lettuce(Lactuca sativa)/InDel marker/polymorphisms/variety identification/cluster analysis分类
农业科技引用本文复制引用
唐丹,涂艺义,黄展倩,肖慧玲,朱雪冰,王昕,陈炯炯..莴苣全基因组InDel标记开发及应用[J].华中农业大学学报,2026,45(2):99-112,14.基金项目
国家自然科学基金项目(32372719) （32372719）
青海省中央引导地方专项(2024ZY041) （2024ZY041）
