渔业研究2026，Vol.48Issue(2)：147-158,12.DOI:10.14012/j.jfr.2025137
卵形鲳鲹G蛋白偶联受体43基因克隆及营养调控分析
Cloning and nutritional regulation analysis of the G-protein-coupled receptor 43(GPR43)gene in Trachinotus ovatus
摘要
Abstract
[Background]Trachinotus ovatus is a tropical-subtropical marine fish species.In recent years,the aquaculture industry of T.ovatus in China has developed rapidly,with key breakthroughs in breeding and cultiv-ation technologies.However,with the expansion of aquaculture scale and the subsequent increase in feed de-mand,optimizing feed formulations to reduce production costs has become a crucial approach for enhancing economic returns.[Objective]This study aims to investigate the role of G-protein-coupled receptor 43(GPR43)gene in energy metabolism in T.ovatus.[Methods]Primers were designed based on the GPR43 gene sequence to identify homologs and clone the coding sequence(CDS).Bioinformatics analysis was subsequently employed to investigate the structural characteristics of the gene and its encoded protein.To further elucidate the functional roles,both in vivo and in vitro experiments were conducted.In vivo,the expression levels of GPR43 gene in ten different tissues of T.ovatus were detected using quantitative real-time polymerase chain reaction(qRT-PCR).Furthermore,the effects of high-sugar feeding and starvation on the expression of this gene in liver and muscle tissues were analyzed.In vitro studies involved isolating primary hepatocytes from T.ovatus liver,which were then subjected to high-sugar and starvation treatments to assess their impact on gene expression.[Results]The CDS of T.ovatus GPR43 gene was 1 011 bp,encoding 336 amino acids,that form a receptor specifically recognizing short-chain fatty acids.Protein structure analysis revealed that T.ovatus GPR43 protein contains seven transmembrane domains.Homology analysis indicated that T.ovatus GPR43 gene shared high homology with GPR43 gene of the same species(amino acid similarity>86%)and was most closely related to T.anak.In contrast,its homology with mammals was relatively low(amino acid similarity was 50%).Tissue dis-tribution analysis showed that GPR43 gene expression was the highest in the liver,followed by the intestine,brain,and muscle,and the lowest in the skin.In vivo results demonstrated that GPR43 gene expression in the liver and muscle of fish fed a high-glucose diet was lower than that in the starvation group.Similarly,in vitro experiments showed that GPR43 gene expression in primary hepatocytes treated with high glucose was lower compared to the starvation group,consistent with the in vivo trend.[Conclusion]The results demonstrate that the expression levels of GPR43 gene in the liver and the muscle of T.ovatus are significantly modulated by diet-ary carbohydrate levels.These findings suggest that GPR43 gene plays a crucial role in sensing energy status and regulating lipid metabolism,thereby serving as a potential biomarker for energy homeostasis in this species.The present study provides valuable insights for reducing feeding costs,controlling body fat content,and im-proving flesh quality in T.ovatus aquaculture.关键词
卵形鲳鲹/GPR43 基因/序列分析/组织分布/营养调控Key words
Trachinotus ovatus/GPR43 gene/sequence analysis/tissue distribution/nutritional regulation分类
农业科技引用本文复制引用
庾娜,陈亚琦,李育浩,王明豪,钟勇,荀鹏伟,李涛,喻大鹏,蔡佳..卵形鲳鲹G蛋白偶联受体43基因克隆及营养调控分析[J].渔业研究,2026,48(2):147-158,12.基金项目
广东省科技计划项目(2025B0202080001) （2025B0202080001）
广东省科技成果入县达镇促进城乡区域协调发展专项项目(2025B0202010041) （2025B0202010041）
农业农村部南海渔业资源开发利用重点实验室开放基金项目(FREU2024-08) （FREU2024-08）
