激光生物学报2026，Vol.35Issue(1)：28-34,52,8.DOI:10.3969/j.issn.1007-7146.2026.01.004
γ射线辐射对质粒DNA损伤的模拟研究
A Simulation Study of Plasmid DNA Damage Caused by Gamma-ray Irradiation
摘要
Abstract
To investigate the early radiochemical processes of γ-ray-induced DNA single-strand breaks(SSB),this study employed the Monte Carlo simulation tool TOPAS and its extended module TOPAS-nBio to construct a water sphere-plasmid microscale simulation model.The model was used to simulate the irradiation of supercoiled pUC19 plasmids by 60Coγ-rays in an aqueous environment and the subsequent induction of SSB in plasmid DNA.The study quantitatively compared the SSB yield under systematically varied conditions of radiation dose,DNA concentration,and hydroxyl radical(·OH)scavenger concentration.The simulation results showed that the SSB yield increased with the elevation of radiation dose.A higher DNA concentration in the environment led to an approximately linear increase in the absolute SSB yield,while the damage efficiency per unit mass of DNA decreased with increasing concentration.An increase in the concentration of·OH scavengers significantly reduced the SSB yield,indicating that the·OH scavenging capacity exerts a marked inhibitory effect on SSB formation.The findings of this study provide certain theoretical support for the optimization of radiotherapy,the formulation of radiation protection strategies,and the health risk assessment of nuclear environments.关键词
伽马射线/质粒DNA/自由基/蒙特卡罗模拟/DNA单链断裂Key words
gamma ray/plasmid DNA/free radical/Monte Carlo simulation/DNA single-strand break分类
数理科学引用本文复制引用
魏凯杰,欧海峰,苗思齐,吕刚..γ射线辐射对质粒DNA损伤的模拟研究[J].激光生物学报,2026,35(1):28-34,52,8.基金项目
河南省自然科学基金项目(252300420319) （252300420319）
河南省科技攻关项目(202102310207) （202102310207）
国家自然科学基金项目(12105076). （12105076）
