激光生物学报2026，Vol.35Issue(1)：88-96,9.DOI:10.3969/j.issn.1007-7146.2026.01.010
穴位贴敷联合加味八珍汤介导的JAK/STAT通路对骨髓抑制小鼠骨髓造血功能的影响
Acupoint Application Combined with Modified Bazhen Decoction Mediated the Effect of JAK/STAT Pathway on Bone Marrow Hematopoietic Function in Mice with Bone Marrow Inhibition
摘要
Abstract
Acupoint application combined with Modified Bazhen Decoction mediated the effect of Janus kinase(JAK)/signal transducer and activator of transcription(STAT)pathway on bone marrow hematopoietic function in mice with bone marrow inhibition.The BALB/c mice were randomly divided into control group,model group,acupoint application group,Modified Bazhen Decoction group,and combined drug group,with 10 mice in each group.Tail vein blood was collected to measure the levels of hemoglobin,white blood cells,and platelets in peripheral blood.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of serum erythropoietin(EPO),thrombopoietin(TPO)and granulo-monocyte colony-stimulating factor(GM-CSF).Bone marrow nucleated cell counts were performed in each group of rats.For the myeloid nucleated cells of each group,colony forming unit-granulocyte and macrophage(CFU-GM),colony forming unit-erythrocyte(CFU-E)and colony forming unit-megakaryocyte,erythrocyte and granulocyte(CFU-Meg)were detected.The kit was used to detect the levels of superoxide dismutase(SOD)and malondialdehyde(MDA)in serum.The expression of p-JAK1,JAK1,p-JAK2,JAK2,p-STAT3,STAT3 proteins in mouse nucleated bone marrow cells were detected by Western blot.The results showed that compared with the control group,the number of hemoglobin,leukocytes,platelets,EPO,TPO,GM-CSF,SOD,bone marrow nucleated cells and the number of cell colonies of hematopoietic progenitor cells CFU-GM,CFU-E and CFU-Meg in the model group decreased(P<0.05),and the MDA,p-JAK1/JAK1,p-JAK2/JAK2,p-STAT3/STAT3 increased(P<0.05).Compared with the model group,the number of hemoglobin,leukocytes,platelets,EPO,TPO,GM-CSF,SOD,bone marrow nucleated cells and the number of cell colonies of hematopoietic progenitor cells CFU-GM,CFU-E and CFU-Meg increased in the acupuncture point application group,Modified Bazhen Decoction group and combined drug group(P<0.05),and the MDA,p-JAK1/JAK1,p-JAK2/JAK2,p-STAT3/STAT3 decreased(P<0.05).Acupoint application and Modified Bazhen Decoction could synergize(P<0.05).It indicated that acupoint application combined with Modified Bazhen Decoction synergistically improves the bone marrow hematopoietic function of mice with bone marrow suppression by reducing oxidative stress,and its mechanism may be related to the inhibition of JAK/STAT signaling pathway.关键词
穴位贴敷/加味八珍汤/骨髓抑制/Janus激酶/信号转导与转录激活因子信号通路/氧化应激Key words
acupoint application/Modified Bazhen Decoction/bone marrow inhibition/Janus kinase/signal transducer and activator of transcription signaling pathway/oxidative stress分类
医药卫生引用本文复制引用
谷大燕,郭进丽,俞永娟,李桂兰..穴位贴敷联合加味八珍汤介导的JAK/STAT通路对骨髓抑制小鼠骨髓造血功能的影响[J].激光生物学报,2026,35(1):88-96,9.基金项目
河北省中医药管理局科研计划项目(2025499). （2025499）
