基础医学与临床2026，Vol.46Issue(4)：484-490,7.DOI:10.16352/j.issn.1001-6325.2026.04.0484
尿液蛋白质组学和代谢组学在慢性低氧模型小鼠中的动态变化
Dynamic changes of urinary proteomics and metabolomics in chronic hypoxia mouse model
摘要
Abstract
Objective To detect changes in the metabolome and proteome of urine in a mouse model of chronic hy-poxia and to explore the correlation between the urinary proteome and metabolome.Methods Six C57BL/6J male mice were placed in an oxygen chamber with 14％oxygen concentration for 5 weeks of hypoxic exposure.Mouse urine was collected weekly with a control group established simultaneously.Non-targeted proteomics and metabolo-mics techniques were employed for dynamic analysis of urine samples.Results Compared to the control group,the urine of chronic hypoxia mice contained 458 differentially expressed proteins and 718 differential metabolites.These proteins and differential metabolites were functionally enriched primarily in glycolysis pathway,lysosomal pathway,and cholesterol synthesis pathway.Joint analysis of proteomics and metabolomics revealed that proteins with high correlation to differential metabolites were mainly enriched in PI3K-AKT,complement and coagulation cascades,and cell cycle pathways.Conclusions Urinary proteomics and metabolomics may reflect the changes induced by chronic hypoxic stimulation.关键词
慢性低氧/尿液/蛋白质组学/代谢组学Key words
chronic hypoxia/urine/proteomics/metabolomics分类
医药卫生引用本文复制引用
刘婷婷,何梦真,张玉雪,胡斯奇,李军,孙伟..尿液蛋白质组学和代谢组学在慢性低氧模型小鼠中的动态变化[J].基础医学与临床,2026,46(4):484-490,7.基金项目
国家自然科学基金(82170524,31901039) （82170524,31901039）
