基础医学与临床2026，Vol.46Issue(4)：524-530,7.DOI:10.16352/j.issn.1001-6325.2026.04.0524
成纤维细胞生长因子受体1杂合缺失损伤小鼠精子活力
Impairment of sperm motility in mice with heterozygous deletion of fibroblast growth factor receptor 1
摘要
Abstract
Objective To investigate the effect of heterozygous deletion of fibroblast growth factor receptor 1(FGFR1)on reproductive function in congenital hypogonadotropic hypogonadism(CHH).Methods A heterozy-gous FGFR1 knockout mouse model(FGFR1+/-)was established.Polymerase chain reaction(PCR)and Sanger sequencing were performed to confirm the deletion of the target exons.Immunohistochemistry and Western blot were used to detect FGFR1 protein expression.Reproductive capacity and function(including sperm concentration,mo-tility,and progressive motility)were evaluated.Additionally,body mass index(BMI),testicular weight,testicular volume,and various sex hormone levels(including T,E2,AMH,FSH,LH,and INH-B)were measured and analyzed statistically.Results The FGFR1 heterozygous knockout mouse model was successfully constructed.PCR and sequencing results confirmed the deletion of exons 4 and 5 of the FGFR1 gene.Western blot and immunohisto-chemistry demonstrated reduced FGFR1 protein expression.Compared with wild-type(WT)mice,FGFR1+/-mice exhibited significantly lower sperm motility(P<0.001)and fewer progressively motile sperm(P<0.01).Compared with WT mice,the INH-B level in FGFR1+/-mice was slightly elevated(P<0.05).Conclusions Heterozygous deletion of FGFR1 impairs sperm motility in mice,providing critical evidence for further elucidating the pathogene-sis of reproductive dysfunction in congenital hypogonadotropic hypogonadism.关键词
成纤维细胞生长因子受体1/精子发生/睾丸发育/先天性低促性腺激素性性腺功能减退症Key words
fibroblast growth factor receptor 1(FGFR1)/spermatogenesis/testicular development/congenital hypogonadotropic hy-pogonadism(CHH)分类
医药卫生引用本文复制引用
李晨阳,李婷,王曦,聂敏,伍学焱,茅江峰,韩钦..成纤维细胞生长因子受体1杂合缺失损伤小鼠精子活力[J].基础医学与临床,2026,46(4):524-530,7.基金项目
北京市自然科学基金(7212080) （7212080）
