基础医学与临床2026，Vol.46Issue(4)：540-546,7.DOI:10.16352/j.issn.1001-6325.2026.04.0540
肉桂酸减轻缺氧/复氧诱导的大鼠心肌细胞系损伤
Cinnamic acid alleviates anoxia/reoxygenation-induced injury of rat myocardial cell line
摘要
Abstract
Objective To explore the mechanism of cinnamic acid(CA)in improving anoxia/reoxygenation(A/R)-induced myocardial cell injury.Methods H9c2 cells were assigned into control group,A/R group,low-dose and high-dose groups of cinnamic acid,HIF-1α activator(DMOG)group,and high-dose cinnamic acid+HIF-1α inhibitor(IDF-11774)group.Cell viability was assessed using the CCK-8 assay.Levels of lac-tate dehydrogenase(LDH)and creatine kinase-MB(CK-MB)in the supernatant were measured by ELISA.Mitochondrial ultrastructure was observed via transmission electron microscopy.Intracellular reactive oxygen species(ROS)and ferrous iron(Fe2+)levels were detected using 2′,7′-dichlorodihydrofluorescein diacetate(DCFH-DA)and FerroOrange fluorescent probes,respectively.Malondialdehyde(MDA)levels and superox-ide dismutase(SOD)activity were determined using commercial kits.Protein expression of HIF-1α,SLC7A11,and GPX4 was analyzed by Western blot.Results Compared with the control group,the A/R group exhibited mitochondrial atrophy,severe cristae disruption,and partial outer membrane rupture.Addi-tionally,cell viability and SOD activity,along with HIF-1α,SLC7A11,and GPX4 protein levels,were sig-nificantly decreased,whereas LDH and CK-MB release,ROS production,MDA levels,and Fe2+accumulation were significantly increased(P<0.05).Compared with the A/R group,the low and high-dose cinnamic acid groups,and DMOG group showed reduced mitochondrial structural injury in H9c2 cells,raised cell viability,SOD activity,HIF-1α,SLC7A11,and GPX4 proteins,and decreased cell supernatant LDH,CK-MB levels,cell ROS fluorescence intensity,MDA and Fe2+fluorescence intensity(P<0.05).IDF-11774 attenuated the inhibitory effect of high-dose cinnamic acid on A/R induced ferroptosis in H9c2 cells and its im-provement effect on cell injury.Conclusions Cinnamic acid alleviates A/R-induced cardiomyocyte injury by inhibiting ferroptosis,likely through activation of the HIF-1α/SLC7A11/GPX4 signaling pathway.关键词
肉桂酸/缺氧/复氧/心肌细胞/铁死亡Key words
cinnamic acid/anoxia/reoxygenation/myocardial cells/ferroptosis分类
医药卫生引用本文复制引用
韩丽婷,王琰,陈星,马佳,刘洋..肉桂酸减轻缺氧/复氧诱导的大鼠心肌细胞系损伤[J].基础医学与临床,2026,46(4):540-546,7.基金项目
郑州市医疗卫生领域科技创新指导计划(2024YLZDJH222) （2024YLZDJH222）
