实用临床医药杂志2026，Vol.30Issue(3)：1-7,15,8.DOI:10.7619/jcmp.20256814
胃癌中免疫相关基因SLC7A7的鉴定与初步验证
Identification and preliminary validation of the immune-related gene SLC7A7 in gastric cancer
摘要
Abstract
Objective To screen novel immune-related genes in gastric cancer through bioinfor-matics approaches,conduct preliminary experimental validation in gastric cancer cells,and elucidate the role of SLC7A7 in the immune microenvironment of gastric cancer via multi-omics integration.Methods Gastric cancer datasets were obtained from the GEO database,and differentially expressed genes were identified.Protein-protein interaction networks were analyzed using STRING software,while gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analy-ses were performed using Metascape.The correlation between gene expression and clinical characteris-tics was validated using TCGA data.The expression of SLC7A7 across various cancers was analyzed u-sing TIMER2.0.Survival analysis was conducted using Kaplan-Meier Plotter.The expression level of SLC7A7 in gastric cancer cell lines was validated by reverse transcription quantitative polymerase chain reaction(RT-qPCR).Results SLC7A7 was highly expressed in gastric cancer and various other canc-ers,and was associated withpoor prognosis.SLC7A7 may exert its effects through the PI3K-Akt and AMPK signaling pathways.SLC7A7 was negatively correlated with memory B cells,naive B cells,monocytes and activated NK cells,while positively correlated with activated CD4+memory Tcells,γδ T cells,resting NK cells,unpolarized macrophages and M1/M2 macrophages.RT-qPCR results demonstra-ted high expression of SLC7A7 in five gastric cancer cell lines(HGC-27,NCI-N87,MKN-45,MKN-74 and AGS).Conclusion SLC7A7 is highly expressed in gastric cancer cell lines and is as-sociated with tumor immune infiltration and poor prognosis in gastric cancer patients.关键词
胃癌/差异表达基因/溶质载体家族7成员7/生物信息学/免疫浸润/肿瘤微环境/预后标志物/信号通路Key words
gastric cancer/differentially expressed gene/solute carrier family 7 member 7/bioin-formatics/immune infiltration/tumor microenvironment/prognostic biomarker/signaling pathway分类
医药卫生引用本文复制引用
李钰,耿进睿,李永红..胃癌中免疫相关基因SLC7A7的鉴定与初步验证[J].实用临床医药杂志,2026,30(3):1-7,15,8.基金项目
国家自然科学基金资助项目(82160534) （82160534）
中央引导地方科技发展基金(25ZYJA003) （25ZYJA003）
甘肃省卫生健康领域重大科技创新项目(GSWSZD2024-01) （GSWSZD2024-01）
甘肃省自然科学基金(23JRRA1491) （23JRRA1491）
甘肃省卫生行业科研项目(GSMSQN2021-004) （GSMSQN2021-004）
