实用临床医药杂志2026，Vol.30Issue(3)：8-15,8.DOI:10.7619/jcmp.20256262
SH2B衔接蛋白3作为胃癌预后生物标志物的生物信息学分析以及实验验证
Bioinformatics analysis and experimental validation of SH2B adaptor protein 3 as a prognostic biomarker for gastric cancer
摘要
Abstract
Objective To investigate the expression of SH2B adaptor protein 3(SH2B3)in gastric cancer cells by combining of bioinformatics methods with experimental validation.Methods RNA-seq data and microarray datasets of gastric cancer from the Gene Expression Omnibus(GEO)and the Cancer Genome Atlas(TCGA)databases were integrated to screen for differentially expressed genes and identify their intersections.Gene Ontology(GO)analysis,Kyoto Encyclopedia of Genes and Ge-nomes(KEGG)pathway enrichment analysis,and protein-protein interaction(PPI)network analysis were performed on the intersecting genes.The expression of SH2B3 and its correlation with clinical characteristics and immune infiltration were explored based on databases,and its expression level in gastric cancer cells was validated by RT-qPCR.Results GO analysis revealed that the genes were primarily involved in processes such as vascular development,extracellular matrix,and oxidoreductase activity.KEGG analysis indicated that the genes were enriched in pathways including the PI3K-Akt pathway,cytokine interactions,and cancer pathways.Gene set enrichment analysis(GSEA)showed that SH2B3 was significantly enriched in epithelial-mesenchymal transition,IL-6/JAK/STAT3 signaling pathway,KRAS-UP signaling pathway,and KRAS-DN signaling pathway.SH2B3 expression was significantly elevated in gastric cancer cells and was associated with poor survival prognosis,with a hazard ratio(HR)of 1.62 for overall survival in patients with high SH2B3 expres-sion.SH2B3 exhibited negative regulation of resting CD4+memory T cells,plasma cells,and acti-vated NK cells,while showing positive regulation of activated CD4+memory T cells and resting NK cells.RT-qPCR analysis revealed high expression of SH2B3 in the gastric cancer cell lines AGS(P＜0.01),HGC-27(P＜0.05),and NCI-N87(P＜0.05).Conclusion SH2B3 is highly ex-pressed in gastric cancer cells and is closely associated with poor prognosis and regulation of the tumor immune microenvironment.SH2B3 may mediate gastric cancer progression through the epithe-lial-mesenchymal transition signaling pathway.关键词
胃癌/差异表达基因/SH2B适配蛋白3/生物标志物/生物信息学/上皮-间质转化/免疫浸润/信号通路Key words
gastric cancer/differentially expressed genes/SH2B adaptor protein 3/biomark-ers/bioinformatics/epithelial-mesenchymal transition/immune infiltration/signaling pathway分类
医药卫生引用本文复制引用
耿进睿,李钰,李永红..SH2B衔接蛋白3作为胃癌预后生物标志物的生物信息学分析以及实验验证[J].实用临床医药杂志,2026,30(3):8-15,8.基金项目
国家自然科学基金资助项目(82160534) （82160534）
中央引导地方科技发展基金(25ZYJA003) （25ZYJA003）
甘肃省卫生健康领域重大科技创新项目(GSWSZD2024-01) （GSWSZD2024-01）
甘肃省自然科学基金(23JRRA1491) （23JRRA1491）
甘肃省卫生行业科研项目(GSMSQN2021-004) （GSMSQN2021-004）
