解放军医学院学报2026，Vol.47Issue(1)：9-18,31,11.DOI:10.12435/j.issn.2095-5227.25101103
粪菌移植对实验性牙周炎小鼠认知功能以及肠道菌群影响的研究
Effects of fecal microbiota transplantation on cognitive function and gut microbiota in experimental periodontitis mice
周虹瑾 1王健辉 2刘琳 3孙巍巍 4李鸿波3
作者信息
- 1. 解放军总医院第一医学中心口腔科,北京 100853||解放军医学院,北京 100853
- 2. 军事科学院军事医学研究院,北京 100850
- 3. 解放军总医院第一医学中心口腔科,北京 100853
- 4. 解放军总医院京西医疗区,北京 100144
- 折叠
摘要
Abstract
Background The gut microbiota may mediate cognitive impairment associated with periodontitis,but there is no direct evidence indicating whether modulating the gut microbiota can ameliorate such impairment.Objective To investigate the effects of fecal microbiota transplantation(FMT)on cognitive function and gut microbiota in experimental periodontitis mice and elucidate the mechanisms by which gut microbiota influences periodontitis-related cognitive decline.Methods C57BL/6 mice were randomly divided into four groups(n=12 per group):control group(C),periodontitis group(P),periodontitis+control FMT group(C-P),and control+periodontitis FMT group(P-C).Experimental periodontitis was induced by silk ligation.After 8 weeks,fecal microbiota suspensions were administered via gavage every other day for 2 weeks.Anxiety-like behavior and cognitive function were assessed using open field test,novel object recognition test,and Y-maze test.Fecal samples were collected for 16S rRNA gene sequencing to analyze changes in gut microbiota.Serum and brain levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)were measured by ELISA.The expression of blood-brain barrier tight junction proteins ZO-1 and Occludin was detected by Western blot.Results Periodontitis induction led to alveolar bone resorption in mice(P＜0.001)and impaired spatial working memory and object recognition(P＜0.05).Meanwhile,serum and brain levels of TNF-α and IL-1β were increased in the periodontitis group(P＜0.05),while the expression of blood-brain barrier tight junction proteins ZO-1 and Occludin was decreased(P＜0.05).16S rRNA sequencing revealed an increased abundance of Muribaculum and Colidextribacter genera in the gut microbiota of periodontitis mice(P＜0.05).FMT treatment improved alveolar bone resorption(P＜0.001)and cognitive function(P＜0.05)in periodontitis mice,reduced inflammatory cytokine levels(P＜0.01),and restored blood-brain barrier integrity(P＜0.05).Conclusion FMT may ameliorate periodontitis-related cognitive decline by modulating gut microbiota balance,reducing systemic and brain inflammation,and repairing blood-brain barrier damage.关键词
牙周炎/16S rRNA/认知功能/粪菌移植/肠道菌群Key words
periodontitis/16S rRNA/cognitive function/fecal microbiota transplantation/gut microbiota分类
医药卫生引用本文复制引用
周虹瑾,王健辉,刘琳,孙巍巍,李鸿波..粪菌移植对实验性牙周炎小鼠认知功能以及肠道菌群影响的研究[J].解放军医学院学报,2026,47(1):9-18,31,11.
