江苏大学学报（医学版）2026，Vol.36Issue(2)：136-141,6.DOI:10.13312/j.issn.1671-7783.y240131
circGFRA1靶向miR-3194-3p调控结直肠癌细胞增殖、迁移及侵袭
circGFRA1 regulates the proliferation,migration and invasion of colorectal cancer cells by targeting miR-3194-3p
摘要
Abstract
Objective:To explore the effect and mechanism of circGFRA1 on the proliferation,migration and invasion of colorectal cancer(CRC)SW620 cells.Methods:qRT-PCR was used to detect the expression levels of circGFRA1 and miR-3194-3p in CRC tissues,adjacent normal tissues,SW620 cells and normal human colonic epithelial NCM460 cells.SW620 cells were cultured in vitro and divided into 6 groups:si-NC group,si-circGFRA1 group,miR-NC group,miR-3194-3p group,si-circGFRA1+anti-miR-NC group,and si-circGFRA1+anti-miR-3194-3p(si-circGFRA1+anti-miR)group.CCK-8 assay and colony formation assay were used to detect cell proliferation ability;scratch assay and Transwell assay were used to detect cell migration and invasion abilities;dual luciferase reporter assay was used to verify the targeting relationship between circGFRA1 and miR-3194-3p;Western blotting was used to detect the protein expression levels of MMP-2 and MMP-9.Results:The expression level of circGFRA1 in CRC tissues was significantly higher than that in adjacent normal tissues(P＜0.001),and the expression level of miR-3194-3p was significantly lower than that in adjacent normal tissues(P＜0.001);the expression level of circGFRA1 in SW620 cells was significantly higher than that in NCM460 cells(P＜0.001),and the expression level of miR-3194-3p was significantly lower than that in NCM460 cells(P＜0.001).Compared with the si-NC group,the cell viability,number of colony formation,migration and invasion cells in the si-circGFRA1 group were significantly reduced(P＜0.05),and the protein expression levels of MMP-2 and MMP-9 were significantly downregulated(P＜0.05);compared with the miR-NC group,the change trends of the above indicators in the miR-3194-3p group were consistent with those in the si-circGFRA1 group(P＜0.05).Dual luciferase reporter assay confirmed that circGFRA1 could specifically bind to miR-3194-3p.Compared with the si-circGFRA1+anti-miR-NC group,the cell viability,number of colony formation,migration and invasion cells in the si-circGFRA1+anti-miR group were significantly increased(P＜0.05),and the protein expression levels of MMP-2 and MMP-9 were significantly upregulated(P＜0.05).Conclusion:circGFRA1 is highly expressed in CRC,and promotes the proliferation,migration and invasion of CRC cells by targeting and inhibiting the expression of miR-3194-3p and upregulating the protein levels of MMP-2 and MMP-9.关键词
结直肠癌/circGFRA1/miR-3194-3p/增殖/迁移/侵袭/基质金属蛋白酶Key words
colorectal cancer/circGFRA1/miR-3194-3p/proliferation/migration/invasion/matrix metalloproteinase分类
医药卫生引用本文复制引用
张荣菊,王汝月,苗志刚,孙荣国,苗玉,周玮玮..circGFRA1靶向miR-3194-3p调控结直肠癌细胞增殖、迁移及侵袭[J].江苏大学学报（医学版）,2026,36(2):136-141,6.基金项目
河北省医学科学研究课题计划项目(20240590) （20240590）
