临床与实验病理学杂志2026，Vol.42Issue(3)：304-309,6.DOI:10.13315/j.cnki.cjcep.2026.03.004
PAIP1在结肠腺癌中的表达及其在细胞增殖中的作用
Expression of PAIP1 in colorectal adenocarcinoma and its role in cell proliferation
摘要
Abstract
Objective To evaluate the expression of PAIP1 in colorectal adenocarcinoma,analyze its correlation with clinicopathological features and prognosis,and identify the role of PAIP1 in the proliferation of colorectal adeno-carcinoma cells.Methods PAIP1 protein expression was detected in 83 cases of colorectal adenocarcinoma tissue and paired adjacent non-tumor tissue using the immunohistochemical SP method.Then,the relationship between PAIP1 protein expression and clinicopathological features of colorectal adenocarcinoma was analyzed.The Kaplan-Meier method was employed to analyze the correlation between PAIP1 protein expression and the survival of colorectal adenocarcinoma patients.The Cox regression model was used to assess the role of PAIP1 in predicting the risk of colorectal adenocarcinoma.Lentivirus-mediated shRNA was used to silence PAIP1 in colorectal adenocarcinoma cells.Then,the effects of PAIP1 silencing on the proliferative ability of colorectal adenocarcinoma cells were deter-mined by CCK-8,EdU,and colony formation assays.Results The positive and strong positive rates of PAIP1 protein in colorectal adenocarcinoma tissues were 92.78%(77/83)and 48.19%(40/83),respectively,significantly higher than in adjacent non-tumor tissues(4.82%,1.20%).Chi-squared test results showed that PAIP1 protein expression was significantly related to the pathological grade(P=0.011).Kaplan-Meier survival analysis indicated that patients with high expression of PAIP1 protein had a significantly lower overall survival than those with low expression(P=0.003 7).Multivariate Cox regression analysis showed that lymph node metastasis(HR=2.918,95%CI=1.405-6.060,P=0.004)and strong positive expression of PAIP1(HR=2.142,95%CI=1.037-4.427,P=0.040)were inde-pendent risk factors for colorectal adenocarcinoma.CCK-8,EdU,and colony formation assay showed that knockdown of PAIP1 inhibited the proliferation capacity of colorectal adenocarcinoma cells.Conclusion PAIP1 is highly ex-pressed in colorectal adenocarcinoma and is closely related to poor prognosis.PAIP1 affects cell proliferation of colorectal adenocarcinoma.关键词
结肠肿瘤/PAIP1/生存分析/预后/增殖Key words
colorectal neoplasms/PAIP1/survival analysis/prognosis/proliferation分类
医药卫生引用本文复制引用
周厚坤,张瑞雪,高媛,金爱花,林贞花,朴俊杰..PAIP1在结肠腺癌中的表达及其在细胞增殖中的作用[J].临床与实验病理学杂志,2026,42(3):304-309,6.基金项目
国家自然科学基金(82060484)、吉林省科技发展计划(20210402029GH) National Natural Science Foundation of China(82060484) （82060484）
Jilin Provincial Science and Technol-ogy Development Program(20210402029GH) （20210402029GH）
