临床与实验病理学杂志2026，Vol.42Issue(3)：325-333,341,10.DOI:10.13315/j.cnki.cjcep.2026.03.007
Rab7a通过Hippo信号通路促进食管癌细胞演进
Rab7a promoted the progression of esophageal cancer cells through the Hippo sig-naling pathway
摘要
Abstract
Objective To investigate the effects of Ras-associated binding protein 7A(Rab7a)on the prolifera-tion and migration of esophageal cancer cells,its relationship with epithelial-mesenchymal transition(EMT),and the underlying mechanisms.Methods Bioinformatics analyses were performed to evaluate Rab7a expression and its prog-nostic value in esophageal cancer,followed by immunohistochemical validation in cancer tissues and normal esopha-geal mucosa.Rab7a was silenced in TE-1 cells using small interfering RNA(siRNA),and knockdown efficiency was verified by real-time quantitative PCR(RT-qPCR)and Western blotting(WB).Cells were divided into a control group,a negative control group(si-NC),and a Rab7a-silenced group(si-Rab7a).CCK-8,EdU,and colony forma-tion assays were conducted to assess cell proliferation.Wound-healing,Transwell,and Hoechst 33342 assays were performed to evaluate migration,invasion,and apoptosis.Gene set enrichment analysis was used to identify key sig-naling pathways regulated by Rab7a in esophageal cancer and EMT,and the results were validated by WB.Results Bioinformatics analyses showed that Rab7a was highly expressed in esophageal cancer and was closely associated with patient prognosis.Immunohistochemistry further confirmed upregulated Rab7a expression in esophageal cancer tis-sues.Functional assays demonstrated that Rab7a silencing inhibited proliferation,migration,and invasion of TE-1 cells and promoted apoptosis.Enrichment analysis identified the Hippo signaling pathway as one of the key pathways regulated by Rab7a.WB further demonstrated that Rab7a knockdown reduced the expression of p-YAP1,YAP1,CTGF,and survivin(P<0.01).In addition,Rab7a silencing upregulated E-cadherin expression while significantly downregulating vimentin and Snail(P<0.01).The expression of the anti-apoptotic protein BCL2 decreased,whereas that of the pro-apoptotic protein Bax increased(P<0.01).Conclusion Rab7a regulated EMT through the Hippo sig-naling pathway,thereby promoting the proliferation,migration,and invasion of esophageal cancer cells.关键词
食管癌/Rab7a/生物信息学/Hippo信号通路Key words
esophageal cancer/Rab7a/bioinformatics/Hippo signaling pathway分类
医药卫生引用本文复制引用
田燕玲,刘博,吕洋..Rab7a通过Hippo信号通路促进食管癌细胞演进[J].临床与实验病理学杂志,2026,42(3):325-333,341,10.基金项目
河北省重点研发计划项目卫生健康创新专项(22377765D)、河北省2024年度医学科学研究指导性课题(20240286) Key Research and Development Program of Hebei Province,Health Innovation Special Project(22377765D) （22377765D）
2024 Guiding Project for Medical Science Research in Hebei Province(20240286) （20240286）
