昆虫学报2026，Vol.69Issue(2)：190-202,13.DOI:10.16380/j.kcxb.2026.02.004
吡虫啉胁迫下斑衣蜡蝉解毒代谢的转录组和代谢组学联合分析
Combined transcriptomic and metabolomic analyses of detoxification metabolisms in Lycorma delicatula(Hemiptera:Fulgoridae)under imidacloprid stress
摘要
Abstract
[Aim]This study aims to lay the foundation for further research on the detoxification mechanisms of insects through identifying the genes and metabolites related to detoxification metabolisms in Lycorma delicatula.[Methods]Indoor toxicity of imidacloprid to adult L.delicatula was determined using the leaf dipping method to definite the sublethal concentration LC10 value and the median lethal concentration(LC50)value in 24 h.Adult L.delicatula treated with LC10 of imidacloprid underwent high-throughput sequencing via the Illumina HiSeq 2000 and TripleTOF 6600+platforms.The nonredundant sequences of genes and metabolites were annotated against NR,Swiss-Prot,Pfam,GO,COG/KOG,KEGG and TrEMBL databases to obtain the annotation information of unigenes.The differentially expressed detoxification enzyme genes and detoxification-related metabolites were screened by DESeq2[with the thresholds of|log2(fold change)|≥1 and false discovery rate(FDR)≤0.05]and PLS-DA[variable important in projection(VIP)＞1,P＜0.05],respectively.The differentially expressed genes(DEGs)and differentially expressed metabolites(DEMs)were annotated against the KEGG database to identify the commonly enriched metabolic pathways,so as to explore the associations between DEGs and DEMs.[Results]The LC10 and LC50 values of imidacloprid to adult L.delicatula in 24 h were 20.799 and 68.707 mg/L,respectively,with the toxicity regression equation of y=-4.533+2.467x.A total of 93 157 unigenes and 179 317 transcripts were obtained by transcriptome sequencing of adult L.delicatula treated with LC10 of imidacloprid.The KEGG annotation result revealed that 168 DEGs involved in 11 metabolic pathways were screened from the transcriptomes between the group treated with LC10 of imidacloprid and the clean water control group,with the DEGs involved in carbohydrate metabolism(50 DEGs)and amino acid metabolism(24 DEGs)being the most prominent.Six detoxification-related DEGs from the cytochrome P450 and UGT families were screened between the group treated with LC10 of imidacloprid and the clean water control group.In addition,134 DEMs were screened from the metabolomes between the group treated with LC10 of imidacloprid and the clean water control group,including 87 down-regulated metabolites and 47 up-regulated metabolites,predominantly benzene derivatives,amino acids and their metabolites,organic acids and their derivatives,heterocyclic compounds,aldehydes,ketones,and esters.[Conclusion]The transcriptome and metabolome databases for adult L.delicatula treated with LC10 of imidacloprid was established,and the key detoxification-related DEGs(for example,cytochrome P450 and UGT genes)and some DEMs were identified.These findings preliminarily elucidate the detoxification mechanisms of L.delicatula,providing a reference for research on the other hemipteran insects and laying a foundation for functional analysis of detoxification enzyme genes and further detoxification mechanism studies of L.delicatula.关键词
斑衣蜡蝉/吡虫啉/解毒酶基因/转录组/代谢物Key words
Lycorma delicatula/imidacloprid/detoxification enzyme genes/transcriptome/metabolites分类
生物科学引用本文复制引用
刘保志,白妍妍,刘欣玉,戚煜,赵盈盈,杨佳婷,吕淑杰,谢寿安..吡虫啉胁迫下斑衣蜡蝉解毒代谢的转录组和代谢组学联合分析[J].昆虫学报,2026,69(2):190-202,13.基金项目
国家公益性行业(林业)科研项目(201504324) （林业）
陕西省第二批特支计划领军人才项目(陕组通字2020-44号) （陕组通字2020-44号）
2022年度西安市科技计划项目(2022 JH-JSYF-0261) （2022 JH-JSYF-0261）
