昆虫学报2026，Vol.69Issue(2)：214-222,9.DOI:10.16380/j.kcxb.2026.02.006
三种虫生真菌对番茄潜叶蛾幼虫的室内毒力测定及温室防治效果
Laboratory virulence assay and greenhouse control efficacy of three entomogenous fungi against the tomato leafminer,Tuta absoluta(Lepidoptera:Gelechiidae)larvae
摘要
Abstract
[Aim]This study aims to clarify the insecticidal effects of three entomogenous fungi against Tuta absoluta,so as to provide a theoretical reference for its biological control.[Methods]Using the topical application method,the laboratory virulence of the spore suspensions of three entomogenous fungi(Lecanicillium lecanii JMC-01,Fusarium equiseti JMF-01,and the UV-mutated strain YB4 of Penicillium raistrickii F-1)at different concentrations(1.0 × 106,1.0 × 107 and 1.0 × 108 cfu/mL)against the 1st-2nd instar larvae of T.absoluta was tested.Based on the laboratory virulence test result,the spore suspensions of the three entomogenous fungi at the concentration of 1.0 × 108 cfu/mL were then used to evaluated their control efficacy against T.absoluta larvae in a greenhouse using a randomized block design.[Results]The laboratory virulence test results demonstrated that all the three entomogenous fungi exhibited significant virulence against the 1st-2nd instar larvae of T.absoluta.At the highest tested concentration(1.0 × 108 cfu/mL),the spore suspensions of L.lecanii JMC-01,the UV-mutated strain YB4 of P.raistrickii F-1 and F.equiseti JMF-01 caused the 5-d cumulative corrected mortality rates of the 1st-2nd instar larvae of T.absoluta to reach 71.79％,61.54％and 58.97％,respectively.The median lethal time(LT50)values of the spore suspensions of L.lecanii JMC-01,the UV-mutated strain YB4 of P.raistrickii F-1 and F.equiseti JMF-01 at the concentration of 1.0 × 108 cfu/mL to the 1st-2nd instar larvae of T.absoluta were 75.5166,89.1429 and 96.4826 h,respectively.In greenhouse control efficacy trials,at the concentration of 1.0 × 108 cfu/mL,L.lecanii JMC-01 demonstrated the control efficacy of 69.28％against T.absoluta larvae at 7 d after the 2nd application,whereas the UV-mutated strain YB4 of P.raistrickii F-1 and F.equiseti JMF-01 achieved the control efficacy of 52.69％and 52.30％,respectively,against T.absoluta at 7 d after the 2nd application.[Conclusion]All the three entomogenous fungi have certain control efficacy against the 1st-2nd instar larvae of T.absoluta larvae,and L.lecanii JMC-01 shows the highest control efficacy,with the potential as a biocontrol agent.关键词
番茄潜叶蛾/虫生真菌/毒力/生物防治/防效Key words
Tuta absoluta/entomogenous fungi/virulence/biological control/control efficacy分类
生物科学引用本文复制引用
田浩然,颜晓曦,李金鹏,白明,王新谱,洪波,贾彦霞..三种虫生真菌对番茄潜叶蛾幼虫的室内毒力测定及温室防治效果[J].昆虫学报,2026,69(2):214-222,9.基金项目
宁夏重点研发计划项目(2023BCF01045) （2023BCF01045）
