中南医学科学杂志2026，Vol.54Issue(2)：201-204,228,5.DOI:10.15972/j.cnki.43-1509/r.2026.02.003
丰富环境上调m6A修饰促进小鼠视皮质突触可塑性相关基因蛋白的表达
Enriched environment upregulates m6A modification and promotes the expression of synaptic plasticity-related genes in the mouse visual cortex
摘要
Abstract
Aim To investigate the effects of enriched environment(EE)on m6A modification and the expression of synap-tic plasticity-related genes and proteins in the mouse visual cortex.Methods Fourteen 8-week-old male C57BL/6J mice were randomly divided into a control group(CON group)and an EE group.Dot blot hybridization and immunohistochemistry were used to detect m6A modification levels in the visual cortex.Western blotting was performed to analyze the expression of methyltransferases and demethylases.qRT-PCR was used to measure mRNA levels of synaptic protein-encoding genes,and Western blotting was applied to detect synaptic protein expression.Results Compared with the CON group,the EE group showed increased m6A modification levels and downregulated expression of the m6A demethylase ALKBH5(P<0.05),while no significant differences were observed in other methylation-related enzymes(P>0.05).Although no significant differences were found in the mRNA levels of synaptic protein genes SYP,PSD-95,and Homer-1 between the two groups(P>0.05),the protein levels of SYP,PSD-95,and Homer-1 were significantly up-regulated in the EE group(P<0.05).Conclusion Enriched environment upregulates m6A modification of plasticity-related genes in the mouse visual cortex,thereby promoting their protein translation and leading to increased expression of synaptic proteins.关键词
丰富环境/视皮质/m6A修饰/突触可塑性/突触蛋白/小鼠Key words
enriched environment/visual cortex/m6A modification/synaptic plasticity/synaptic proteins/mouse分类
医药卫生引用本文复制引用
张文瑶,陈金莺,钟敬祥..丰富环境上调m6A修饰促进小鼠视皮质突触可塑性相关基因蛋白的表达[J].中南医学科学杂志,2026,54(2):201-204,228,5.基金项目
国家自然科学基金项目(81970806,82271094) （81970806,82271094）
广州市科技计划项目(202201020030,202201020015,202201010618) （202201020030,202201020015,202201010618）
广东省医学科研基金项目(A2022415) （A2022415）
