中南医学科学杂志2026，Vol.54Issue(2)：216-220,5.DOI:10.15972/j.cnki.43-1509/r.2026.02.006
沉默P4HA1对结直肠癌HT-29、SW620细胞增殖、侵袭的影响机制
The mechanism of silencing P4HA1 to influence the proliferation and invasion of HT-29 and SW620 colorectal cancer cells
摘要
Abstract
Aim To explore the mechanism of silencing P4HA1 on the proliferation and invasion of colorectal cancer(CRC)cells HT-29 and SW620.Methods The expression level of P4HA1 in CRC tissues and its relationship with patient prognosis were analyzed through the GEPIA and Kaplan-Meier Plotter databases.Immunohistochemistry was used to detect the expression of P4HA1 in cancerous and adjacent normal tissues from 90 CRC patients.The proliferation,invasion,and migration abilities of HT-29 and SW620 cells after P4HA1 silencing were assessed using cell proliferation experiments,plate cloning experiments,and Transwell experi-ments.Western blotting was employed to detect the expression levels of epithelial-mesenchymal transition(EMT)-related proteins[E-cadherin,N-cadherin,and vimentin]in SW620 cells after P4HA1 silencing.Results The expression level of P4HA1 in CRC tissues was higher than that in normal tissues adjacent to the cancerous tissues.High expression of P4HA1 was a poor prognostic factor for overall survival in CRC patients(P<0.05).After silencing P4HA1,the proliferation,invasion,and migration abilities of CRC HT-29 and SW620 cells were significantly decreased(P<0.05).After silencing P4HA1,the expression of N-cadherin and vi-mentin in CRC SW620 cells were downregulated(P<0.05),while the expression of E-cadherin was upregulated(P<0.05).Conclusion Silencing P4HA1 inhibits the proliferation and invasion abilities of CRC HT-29 and SW620 cells,and its mechanism may be related to regulating the EMT process.关键词
沉默P4HA1/CRC/HT-29细胞/SW620细胞/细胞增殖/细胞侵袭Key words
silence P4HA1/CRC/HT-29 cells/SW620 cells/cell proliferation/cell invasion分类
医药卫生引用本文复制引用
陈志杰,李缘,邓祖亮,胡杨志..沉默P4HA1对结直肠癌HT-29、SW620细胞增殖、侵袭的影响机制[J].中南医学科学杂志,2026,54(2):216-220,5.基金项目
国家级大学生创新创业训练计划项目(202210545019) （202210545019）
湖南省自然科学基金项目(2023JJ50067) （2023JJ50067）
湖南省教育厅科学研究项目(21B0749) （21B0749）
湖南省大学生创新创业训练计划项目(3732) （3732）
