中国海洋大学学报（自然科学版）2026，Vol.56Issue(4)：39-48,99,11.DOI:10.16441/j.cnki.hdxb.20250145
凡纳滨对虾组织中虾青素的分析方法建立、分布特征及功能探究
Establishment of Analytical Method,Distribution Characteristics and Functional Investigation of Astaxanthin in Tissues of Litopenaeus vannamei
摘要
Abstract
To elucidate the distribution characteristics of astaxanthin in Pacific white shrimp(Litopenaeus vannamei),a robust method for the extraction and quantification of astaxanthin in shrimp tissues was developed.Methanol was identified as the optimal extraction solvent.A single-step extraction at a 1:50(w/v)solid-liquid ratio combined with a methanol-THF sequential dissolution approach was utilized.Through controlled feeding trials with graded astaxanthin supplementation(0,40,80,120 mg/kg),we quantitatively analyzed the tissue-specific accumulation patterns,body color responses and associated enzymatic changes.The results indicated that astaxanthin significantly enhanced the body color of shrimp to a red-orange hue and was predominantly accumulated in the hepatopancreas,ventral nerve cord and epidermis.Additionally,astaxanthin supplementation effectively increased the total antioxidant capacity(Total antioxidant capacity,T-AOC)of the hepatopancreas,elevated the activities of superoxide dismutase(Superoxide dismutase,SOD),catalase(Catalase,CAT),alkaline phosphatase(Alkaline phosphatase,AKP)and glutathione peroxidase(Glutathione peroxidase,GSH-Px),and reduced the content of malondialdehyde(Malondialdehyde,MDA).These findings demonstrated that astaxanthin significantly enhances the antioxidant capacity and immune function of L.vannamei.关键词
凡纳滨对虾/虾青素/定量方法/抗氧化/免疫功能Key words
Litopenaeus vannamei/astaxanthin/quantitative method/antioxidant/immune function分类
生物科学引用本文复制引用
贾佳,刘怡凤,赵倩倩,孙玥,包振民..凡纳滨对虾组织中虾青素的分析方法建立、分布特征及功能探究[J].中国海洋大学学报（自然科学版）,2026,56(4):39-48,99,11.基金项目
山东省优秀青年科学基金项目(2023HWYQ-066) （2023HWYQ-066）
中央高校基本科研业务费项目(202312036)资助Supported by the Shandong Excellent Young Scientists Fund Program(2023HWYQ-066) （202312036）
the Fundamental Research Funds for the Central Universities(202312036) （202312036）
