人民长江2026，Vol.57Issue(3)：8-15,8.DOI:10.16232/j.cnki.1001-4179.2026.03.002
丹江口水库2-甲基异莰醇来源解析及产嗅潜力评估
Source apportionment and odor-producing potential assessment of 2-methylisoborneol in Danjiangkou Reservoir
摘要
Abstract
In recent years,odor problems caused by 2-methylisoborneol(2-MIB)have been frequently reported,posing a prominent challenge to global water quality safety management.Monitoring data showed that in mid-to-late August 2025,the average concentration of 2-MIB at the Taocha Section of the main channel of the South-to-North Water Diversion Middle Route Project reached 29.0 ng/L.To elucidate the biological sources and odor-producing potential of 2-MIB,this study focused on a period of abnormal algal proliferation in the Danjiangkou Reservoir.Methods including morphological identification via mi-croscopy,high-throughput sequencing of the mic gene,and quantitative PCR(qPCR)were comprehensively employed to system-atically analyze the cyanobacterial community structure,potential odor-producing taxa,and the spatiotemporal distribution char-acteristics of mic gene abundance.Morphological identification revealed a cyanobacterial community dominated by Aphanocapsa,Raphidiopsis,and Leptolyngbya.Molecular biological methods further uncovered numerous ecologically significant taxa with low a-bundance.Planktothricoides and Pseudanabaena were identified as the main potential odor-producing groups.Among these,Planktothricoides accounted for 64.2%～93.3%of the mic gene-carrying community and showed a significant positive corre-lation with mic gene abundance,leading to the inference that it was the primary contributor to 2-MIB.The mic gene copy num-bers ranged from 3.14×105to 2.00×107 copies/L,exhibiting significant spatial differences and vertical stratification,with the highest abundance found in the surface water of Taocha Section.This study confirms that combining morphological and molecular biological methods can effectively enhance the accuracy of identifying odor-producing algae and assessing associated risks,there-by providing a scientific basis for the early warning and control of 2-MIB risks in drinking water sources.关键词
2-甲基异莰醇/嗅味物质/定量PCR/16S rRNA测序/mic基因/丹江口水库Key words
2-methylisoborneol/taste and odor compounds/qPCR/16S rRNA/mic gene/Danjiangkou Reservoir分类
资源环境引用本文复制引用
赵媛,刘畅,雷欢,高广斌,陈威,马沛明..丹江口水库2-甲基异莰醇来源解析及产嗅潜力评估[J].人民长江,2026,57(3):8-15,8.基金项目
国家重点研发计划项目(2024YFC3210901) （2024YFC3210901）
国家自然科学基金青年基金项目(52200231) （52200231）
