上海预防医学2026，Vol.38Issue(2)：139-145,7.DOI:10.19428/j.cnki.sjpm.2026.250256
围产期双酚AF暴露对子代雄性大鼠青春前期的致肥作用
Obesogenic effects of perinatal bisphenol AF exposure on prepubertal male offspring rats
摘要
Abstract
[Objective]To investigate the disruptive effects of perinatal exposure to the environmental endocrine disruptor bisphenol AF(BPAF)on hepatic lipid metabolism in prepubertal(postnatal day 21,PND21)male offspring rats,and to provide scientific evidence for assessing the obesogenic effect of BPAF.[Methods]Sprague-Dawley(SD)rats aged 8 weeks were used in this study.Pregnant rats were divided into BPAF dose groups(2,10,50 mg·kg⁻¹)and a vehicle control group(corn oil),with 6 confirmed pregnant females per group.Gavage administration started from gestational day 0 and continued until the end of lactation.At PND21,one male offspring per litter was randomly selected.Serum concentrations of glucose(GLU),triglyceride(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),leptin(LEP),free fatty acid(FFA),as well as oxidative stress markers superoxide dismutase(SOD)and malondialdehyde(MDA),were measured.Pathological changes in liver and adipose tissues were evaluated,and the expression levels of genes related to hepatic lipid metabolism were measured.[Results]Compared to the vehicle control group,the 50 mg·kg⁻¹ group showed significantly increased serum LEP and MDA levels in male offspring(P<0.05),and significant upregulation of hepatic lipoprotein lipase(Lpl),fatty acid synthetase(Fas),and peroxisome proliferator-activated receptor γ(Pparg)gene expression(P<0.05).The 2 mg·kg⁻¹ group exhibited a significant increase in adipocyte length(P<0.05),while the 50 mg·kg⁻¹ group showed significant increases in both adipocyte area and length(P<0.05).No significant abnormalities were observed in liver histopathological examination.[Conclusion]Perinatal exposure to 50 mg·kg⁻1 BPAF induced adipocyte hypertrophy,elevated leptin levels,upregulation of lipid synthesis gene expression,and enhanced oxidative stress in prepubertal male offspring,suggesting that BPAF may exert environmental obesogenic effects by disrupting lipid metabolism pathways.关键词
双酚AF/围产期暴露/致肥作用/脂质代谢/环境致肥因子Key words
bisphenol AF/perinatal exposure/obesogenic effect/lipid metabolism/environmental obesogen分类
医药卫生引用本文复制引用
刘玺,杨正丽,成雪晴,杨隽,徐菁,洪新宇..围产期双酚AF暴露对子代雄性大鼠青春前期的致肥作用[J].上海预防医学,2026,38(2):139-145,7.基金项目
上海市自然科学基金项目(23ZR1459600) （23ZR1459600）
上海市卫生健康委员会科研项目(20224Y0329) （20224Y0329）
