热带亚热带植物学报2026，Vol.34Issue(2)：215-223,9.DOI:10.11926/jtsb.4999
基于AFLP标记技术的芒果种质资源遗传多样性分析
Genetic Diversity Analysis of Mangifera indica Germplasm Resources Based on AFLP Marker Technology
摘要
Abstract
Mangifera indica germplasm resources serve as the foundation for mango breeding and are of great significance for the development of new varieties and the conservation of genetic diversity.The genetic diversity and cluster analysis on 128 mango germplasm resources preserved in the Yunnan Innovation Base for Mango Germplasm Resources Conservation of Ministry of Agriculture and Rural Affairs were studied by using amplified fragment length polymorphism(AFLP)technology.Five highly polymorphic primer pairs were selected from 64 pairs for fluorescence AFLP detection,and a total of 255 loci were identified,among which 245 were polymorphic loci,with a polymorphism rate of 96.08%.Genetic parameter calculations for all loci revealed an average observed allele number of 1.967 3,an average effective allele number of 1.573 3,an average Nei's gene diversity index of 0.345 8,and an average Shannon's diversity index of 0.517 9,indicating that the 128 germ-plasm resources possess rich genetic diversity.Based on the genetic similarity coefficient,cluster analysis divided the 128 germplasm resources into three major categories at a genetic similarity coefficient of 0.77.The first category included 95 resources,the second category 24 resources,and the third category 9 resources.Moreover,there was a phenomenon of cross-clustering among germplasm resources of different pedigrees.Furthermore,high genetic similarity was detected between unknown59 and Purple Flower Mango,Creamy Fragrant Mango and unknown27,as well as unknown10 and unknown25.These would provide an important basis for the selection and utilization of mango germplasm in the future,and offer scientific references for genetic breeding,germplasm conservation,and sustainable use of mango resources.关键词
芒果/种质资源/AFLP/遗传多样性/聚类分析Key words
Mangifera indica/Germplasm resources/AFLP/Genetic diversity/Cluster analysis引用本文复制引用
毛常丽,刘紫艳,龙青姨,郑诚,柳觐,牛迎凤..基于AFLP标记技术的芒果种质资源遗传多样性分析[J].热带亚热带植物学报,2026,34(2):215-223,9.基金项目
云南省基础研究计划重点项目(202501AS070159) （202501AS070159）
云南省技术创新人才培养对象项目(202305AD160023) （202305AD160023）
西双版纳州内科研院所科技创新项目(科技创新202401011) （科技创新202401011）
云南省所热带作物科技创新专项资金(RF2025)资助 This work was supported by the Key Project for Basic Research in Yunnan(Grant No.202501AS070159),the Project for Technology Innovation Talent Training in Yunnan(Grant No.202305AD160023),the Project for Scientific and Technological Innovation of Scientific Research Institutes in Xishuangbanna(Grant No.202401011),and the Special Project for Scientific and Technological Innovation of Tropical Crops in Yunnan(Grant No.RF2025). （RF2025）
