农业生物技术学报2026，Vol.34Issue(4)：685-696,12.DOI:10.3969/j.issn.1674-7968.2026.04.001
水稻F3H基因的同源克隆及表达分析
Homologous Cloning and Expression Analysis of F3H Gene in Oryza sativa
摘要
Abstract
Flavonoids play a critical role in plant resistance to various biotic and abiotic environmental stresses.Guizhou province is located in the subtropical monsoon climate zone with abundant but uneven precipitation,where spring drought frequently occurs and severely affects rice(Oryza sativa)sowing and seedling growth.In this study,the flavanone 3-hydroxylase(OsF3H)gene(GenBank No.XM_015776658)was cloned from rice leaves.Its CDS was 1 041 bp in length,encoding 346 amino acids,and the predicted protein molecular weight was 38.74 kD.Phylogenetic analysis revealed that OsF3H clusters with flavanone 3-hydroxylases from other species,indicating it was a member of the flavanone 3-hydroxylase family and shared the closest relationship with wheat(Triticum aestivum)F3H.Cis-acting element prediction indicated that the promoter region of this gene contained not only functional elements responsive to phytohormone signals,light regulation,as well as growth and development,but also cis-acting elements involved in drought and salt stress responses.Further expression analysis under drought treatment demonstrated that OsF3H could be induced by drought,suggesting its potential role in drought stress response of rice.To verify its function in drought response,OsF3H was heterologously expressed in Arabidopsis thaliana,and the transgenic plants were subjected to drought stress.The results showed that the transgenic plants exhibited significantly increased root length,greening rate,fresh weight,and dry weight relative to the wild-type plants,indicating that overexpression of OsF3H significantly enhanced drought tolerance of A.thaliana.These findings provide important data for further elucidating the physiological function of OsF3H in rice and its mechanism in response to drought stress,which will facilitate subsequent research.关键词
水稻/F3H/抗逆/生物信息学分析Key words
Oryza sativa/F3H/Stress resistance/Bioinformatics analysis分类
农业科技引用本文复制引用
舒合凤,胡荣美,杨林荣,郭慧,甘雨,王倩,朱速松,孙威..水稻F3H基因的同源克隆及表达分析[J].农业生物技术学报,2026,34(4):685-696,12.基金项目
贵州省科技计划项目(黔科合支撑[2022]重点028) （黔科合支撑[2022]重点028）
