农业生物技术学报2026，Vol.34Issue(4)：719-732,14.DOI:10.3969/j.issn.1674-7968.2026.04.004
葡萄CLE基因家族鉴定及表达分析
Identification and Expression Analysis of CLE Gene Family in Grape(Vitis vinifera)
摘要
Abstract
CLAVATA3/embryo surrounding region-related(CLE)genes are a class of gene widely distributed in plants that encode signaling peptides responses.In this study,CLE gene family members were identified from the whole genome of grape(Vitis vinifera)using bioinformatic approaches.Their physicochemical properties,phylogenetic relationships,gene structures and conserved motifs,chromosomal localization,and promoter cis-acting elements were systematically analyzed.At the same time,the expression levels of gibberellin(GA3)induced gibberellin in different organs were compared by combining transcriptome and qRT PCR.The results showed that a total of 15 CLE gene family members were present in the grape genome;they were unevenly distributed on 8 chromosomes,encoded proteins of 78～137 amino acids,and were classified into 4 subgroups.Subcellular localization predictions indicated that most VvCLE proteins were localized in the chloroplasts and nucleus,and all members contained the conserved CLE motif.Analysis of the promoter regions of VvCLE genes revealed multiple cis-acting elements associated with growth and development,hormone responses,light responsiveness,and stress adaptation.VvCLE genes were expressed in different organs of grape and exhibited obvious tissue specificity among them,VvCLE6 was highly expressed in the fruit flesh and was significantly induced by GA3,which was consistent with transcriptome data and suggested that it may be involved in fruit development.This study provides valuable resources and a theoretical basis for functional characterization and regulatory mechanism studies of the CLE gene family in grape.关键词
葡萄/CLAVATA3/胚胎周围区域(ESR)相关(CLE)基因家族/表达分析/赤霉素Key words
Grape/CLAVATA3/Embryo surrounding region(ESR)-related(CLE)gene family/Expression analysis/Gibberellin分类
农业科技引用本文复制引用
郑倩倩,胡玲玲,房聪玲,王立如,周书怡,张丹艺蝶,吴月燕,杨中义..葡萄CLE基因家族鉴定及表达分析[J].农业生物技术学报,2026,34(4):719-732,14.基金项目
宁波市公益性研究计划重点项目(2024S016) （2024S016）
浙江"生物工程"一流学科自设课题(ZS2023016) （ZS2023016）
浙江省大学生科技创新活动计划暨新苗人才计划(2024R420A013) （2024R420A013）
浙江省"生物工程"一流学科学生创新计划(CX2024047) （CX2024047）
