饲料研究2026，Vol.49Issue(4)：73-78,6.DOI:10.13557/j.cnki.issn1002-2813.2026.04.013
基于响应面优化中药单体组合及其对仔猪腹泻产肠毒素大肠杆菌的抑菌作用
Optimization combination of Chinese medicine monomer combination based on response surface methodology and its antibacterial effect against enterotoxigenic Escherichia coli in piglets with diarrhea
摘要
Abstract
This study aimed to optimize the composite ratio of three Chinese medicine monomers-quercetin,baicalin,and berberine-using response surface methodology and to evaluate their antibacterial effect against enterotoxigenic Escherichia coli(ETEC)causing piglet diarrhea.The minimum inhibitory concentration(MIC)of each monomer against ETEC was determined by the microbroth dilution method,and the combined effect was assessed using the fractional inhibitory concentration index(FICI).The optimal mass ratio of the monomers was obtained through response surface optimization using response surface methodology.The effects of the optimized monomer combination on bacterial growth curve,conductivity,alkaline phosphatase(AKP)activity,and DNA fluorescence signal were measured.The results showed that the MIC of quercetin,baicalin,and berberine against ETEC was 5.00,3.00,and 3.75 g/L,respectively.FICI tests indicated that the combined effects of these monomers were additive.Response surface optimization revealed the optimal mass ratio of quercetin:baicalin:berberine to be 1∶2∶2.Compared with the control group,bacterial growth in all treatment groups was inhibited.The conductivity and AKP activity in the treatment groups were significantly higher than those in the control group(P<0.05),with the 2 MIC group showing significantly higher conductivity than the MIC and 0.5 MIC groups(P<0.05).The DNA fluorescence signal in the treatment groups was significantly lower than that in the control group(P<0.05).The study shows that the optimized Chinese medicine monomer combination exerts its antibacterial effect by disrupting the integrity of the cell wall and cell membrane of ETEC and affecting its genetic material,providing a reference for developing novel herbal preparations as alternatives to antibiotics.关键词
中药单体物质/产肠毒素大肠杆菌/响应面优化/抗菌Key words
Chinese medicine monomers/enterotoxigenic Escherichia coli/response surface optimization/antibacterial分类
农业科技引用本文复制引用
刘雪松,江波涛,张艳,丁嘉烽,孟维珊,薛沾枚,王爽,吴宪..基于响应面优化中药单体组合及其对仔猪腹泻产肠毒素大肠杆菌的抑菌作用[J].饲料研究,2026,49(4):73-78,6.基金项目
黑龙江省省属科研院所科研业务费(项目编号:CZKYF2025-1-B011) （项目编号:CZKYF2025-1-B011）
"十四五"国家重点研发计划项目(项目编号:2024YFD1301303) （项目编号:2024YFD1301303）
