饲料研究2026，Vol.49Issue(3)：17-23,7.DOI:10.13557/j.cnki.issn1002-2813.2026.03.004
甘草酸对湖羊生长性能、抗氧化功能、免疫力和肠道健康的影响
Effects of glycyrrhizic acid on growth performance,antioxidant function,immunity,and intestinal health in Hu sheep
摘要
Abstract
This study aimed to investigate the antibacterial effect of glycyrrhizic acid and its effects on the growth performance,antioxidant capacity,immune function,and intestinal health of Hu sheep.The punch method was used to determine the inhibitory effect of glycyrrhizic acid on the growth of pathogenic Escherichia coli,Staphylococcus aureus,and Salmonella typhimurium.A total of 60 healthy Hu sheep with an initial body weight of(15.95±0.45)kg were randomly divided into four groups,with three replicates per group and five sheep per replicate.The control group was fed a basal diet,while experimental groups Ⅰ,Ⅱ,and Ⅲ received the basal diet supplemented with 2,4,and 8 g/kg glycyrrhizic acid,respectively.The pre-test period was seven days,and the formal test period was 60 days.The results showed that glycyrrhizic acid could effectively inhibit the growth of pathogenic Escherichia coli and Staphylococcus aureus.Compared with the control group,the final body weight of experimental groups Ⅱ and Ⅲ was significantly increased(P<0.05).In experimental group Ⅱ,serum total antioxidant capacity(T-AOC)and superoxide dismutase(SOD)activity were significantly increased(P<0.05),while malondialdehyde(MDA)content was significantly decreased(P<0.05).The glutathione peroxidase(GSH-Px)activity in serum was significantly increased in experimental groups Ⅱ and Ⅲ(P<0.05).The serum levels of interleukin-4(IL-4),interleukin-10(IL-10),immunoglobulin G(IgG),immunoglobulin A(IgA),and the ileal mucus secretory immunoglobulin A(SIgA)were significantly elevated in experimental groups Ⅱ and Ⅲ(P<0.05),while interleukin-1β(IL-1β)and interleukin-6(IL-6)levels were significantly reduced(P<0.05).The mRNA relative expression levels of Occludin,zonula occludens-1(ZO-1),α-catenin,and mucin 2(Muc2)in the colon were significantly increased in experimental groups Ⅱand Ⅲ(P<0.05).The mRNA relative expression levels of ZO-1 and Muc2 in experimental group Ⅲ were significantly higher than in experimental group Ⅱ(P<0.05).The fecal Escherichia coli count in experimental groups Ⅱ and Ⅲ was significantly lower than in the control group(P<0.05).The study shows that dietary supplementation with 4 g/kg and 8 g/kg glycyrrhizic acid can promote growth,enhance antioxidant capacity and immune function,upregulate the expression of intestinal tight junction proteins,and reduce fecal Escherichia coli counts in Hu sheep.关键词
甘草酸/湖羊/生长/抗氧化/免疫/肠道健康Key words
glycyrrhizic acid/Hu sheep/growth/antioxidant/immunity/intestinal health分类
农业科技引用本文复制引用
郑殿重,刘玉峰,李满雨,何鑫淼,柴孟龙,刘娣..甘草酸对湖羊生长性能、抗氧化功能、免疫力和肠道健康的影响[J].饲料研究,2026,49(3):17-23,7.基金项目
黑龙江省农业科技创新特色产业项目(项目编号:CX23TS20) （项目编号:CX23TS20）
博士科研启动金项目(项目编号:CX23BS02) （项目编号:CX23BS02）
