生物技术通报2026，Vol.42Issue(2)：77-88,12.DOI:10.13560/j.cnki.biotech.bull.1985.2025-1382
增温下直接种间电子转移对泥炭地甲烷产生的作用
Role of Direct Interspecies Electron Transfer in the Methane Production of the Peatland under Warming
摘要
Abstract
[Objective]To explore the role of direct interspecies electron transfer(DIET)mechanisms in methane production from the Zoige peatland on the Qinghai-Tibet Plateau under warming conditions,and to provide DIET-based methane mitigation strategies in peatlands under global warming.[Method]Soils growing three dominant plant species in the Zoige peatland on the Tibetan Plateau were sampled to construct methanogenic and electrochemical enrichments,which were amended with pectin or cellulose and incubated at 18℃.By adding biochar(as an electron transfer shuttle)and electrochemical experiments,the effects of DIET on methane production from plant organic complexes and its response to warming(28℃)were investigated.[Result]The addition of biochar into the soil enrichments elevated methane production from cellulose and pectin by 1.1 to 9.8-fold and increased maximum methanogenic rates by 1.4 to 12.3-fold,and the temperature sensitivity of methanogenesis was doubled following biochar addition.Chronoamperometry detected extracellular electrons generated by soil bacteria in the electrochemically enriched cultures,with a current density of 33.7 μA/cm2,and current consumption coupled methanogenesis by methanogenic archaea(21.2 μA/cm2)in electrochemical enrichments.16S rRNA gene diversity analysis revealed that biochar selectively enriched the electroactive bacterial family Geobacteraceae,along with methanogenic archaea communities including the families Methanosarcinaceae,Methanomethylophilaceae(electroactive uncharacterized),and the uncultured methanogen group Rice Cluster Ⅱ.Co-occurrence network analysis further indicated significantly positive correlations between Geobacteraceae or Cellulomonadaceae and Rice Cluster Ⅱ,suggesting that they implemented DIET-based conversion of the plant organic complexes to methane.[Conclusion]DIET plays a main role in methane production from plant organic complexes in Zoige peatland,particularly under the warming scenarios,thereby inhibiting interspecies electron transfer should be considered as one of the key strategies for methane mitigation in peatlands.关键词
低温泥炭地/甲烷产生/电活性微生物/电子转移/增温Key words
cold peatland/methane production/electroactive microorganisms/electron transfer/warming引用本文复制引用
王雪萌,东秀珠,薛凯,李凌燕..增温下直接种间电子转移对泥炭地甲烷产生的作用[J].生物技术通报,2026,42(2):77-88,12.基金项目
国家自然科学基金面上项目(32570125),国家重点研发计划(2025YFF0512900,2024YFF0808702),地球多圈层相互作用的油气富集理论(THEMSIE04010106) （32570125）
