生物技术通报2026，Vol.42Issue(2)：102-112,11.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0719
回流比对厌氧氨氧化系统的影响及作用机制研究
Study on the Influence and Mechanism of Reflux Ratio on Anaerobic Ammonia Oxidation System
摘要
Abstract
[Objective]To explore the effect of reflux ratio on denitrification performance of anaerobic ammonia oxidation system and reveal its mechanism of action.[Method]Three reflux ratios(0%,50%,and 100%)were set to determine the denitrification performance,granular sludge characteristics,microbial community structure,and nitrogen metabolism related functional genes of the anaerobic ammonia oxidation system at different reflux ratios.[Result]When the reflux ratio was 100%,the average total nitrogen removal efficiency and the average NRR(nitrogen removal rate)during the stable phase of the system increased by 9.32%and 36.09 mg/(L‧d),respectively,with more stable nitrogen removal performance.The ratios of ∆NO₂--N/∆NH₄⁺-N and ∆NO₃--N/∆NH₄⁺-N were closer to the theoretical values of anammox.The effluent had a lower oxidation-reduction potential,which improved the oxygen environment in the system.The color of granular sludge was redder,the degree of granulation increased,and the phenomenon of upward floating significantly reduced.The contents of tightly bound polysaccharides and loosely bound polysaccharides within the granules increased by 22.59 and 24.00 mg/g VSS,respectively,forming a more stable shear-resistant structure.The increase of C-O and C=C functional groups on the surface of granular sludge accelerated the electron transfer in the system.High-throughput sequencing showed that Candidatus Brocadia maintained a high relative abundance(38.61%),while the relative abundance of Denitratisoma increased by 4.13%.Meanwhile,nitrogen metabolism-related functional genes narG,napA,narZ and nirS were upregulated[Conclusion]An appropriate reflux ratio can effectively improve the denitrification performance and operational stability of anaerobic ammonia oxidation systems.关键词
回流比/厌氧氨氧化/颗粒污泥/菌群结构/功能基因/作用机制Key words
reflux ratio/anammox/granule sludge/microbial community structure/functional gene/mechanism of action引用本文复制引用
陈仔龙,张超,郝宛婷,宋贤威,陈欣怡,卢璐,李鹏飞,朱易春..回流比对厌氧氨氧化系统的影响及作用机制研究[J].生物技术通报,2026,42(2):102-112,11.基金项目
国家自然科学基金项目(52260004),江西理工大学高层次人才科研启动项目(205200100642),江西理工大学研究生创新专项资金项目(YC2025-S579,XY2024-S152) （52260004）
