水生生物学报2026，Vol.50Issue(4)：19-28,10.DOI:10.3724/1000-3207.2025.2025.0386
人工繁育环境中长江鲟繁殖活动的环境DNA动态研究
ENVIRONMENTAL DNA DYNAMICS DURING REPRODUCTIVE ACTIVITY OF THE ACIPENSER DABRYANUS IN CAPTIVE BREEDING SYSTEMS
摘要
Abstract
We applied digital PCR targeting mitochondrial DNA(mtDNA)and nuclear DNA(nuDNA)markers to investigate environmental DNA(eDNA)dynamics during artificial propagation of the critically endangered Yangtze sturgeon(Acipenser dabryanus).Species-specific primers and TaqMan probes were designed to quantify mtDNA and nuDNA concentrations and their ratio(nuDNA/mtDNA)across different reproductive stages.Both mtDNA and nuDNA levels increased rapidly and reached their peaks after spawning.In the male pond,concentrations rose to 27-fold(mtDNA)and 130-fold(nuDNA)of pre-spawning levels following sperm release;in the female pond,concentra-tions increased to 8-fold(mtDNA)and 13-fold(nuDNA)after ovulation.The nuDNA/mtDNA ratio peaked at 0.14 and 0.09 in the male and female ponds,respectively.Overall,peak mtDNA concentrations were approximately 11-12 times higher than those of nuDNA and remained detectable for 24-36h,indicating greater persistence.In contrast,nuDNA concentrations declined sharply within 12h post-spawning,demonstrating high temporal sensitivity to spawning events.Our findings demonstrate that eDNA monitoring effectively captures molecular signatures and temporal patterns of A.dabryanus reproductive activity,offering a robust,non-invasive tool for studying the reproductive ecology and inform-ing conservation strategies for endangered fish species.关键词
环境DNA/繁殖/数字PCR/mtDNA/nuDNA/长江鲟Key words
Environmental DNA/Spawning/Digital PCR/mtDNA/nuDNA/Acipenser dabryanus分类
农业科技引用本文复制引用
申晓雪,满李君,刘青娟,李莎,刘焕章,俞丹..人工繁育环境中长江鲟繁殖活动的环境DNA动态研究[J].水生生物学报,2026,50(4):19-28,10.基金项目
科技基础资源调查专项(2022FY100400) （2022FY100400）
三峡工程运行安全综合监测系统,库区维护和管理基金(2136703)资助[Supported by the National Science and Technology Basic Resources Survey Special Project(2022FY100400) （2136703）
the Comprehensive Safety Monitoring System of Three Gorges Project,Reservoir Operation and Management(2136703)] （2136703）
