生物技术通报2026，Vol.42Issue(2)：149-157,9.DOI:10.13560/j.cnki.biotech.bull.1985.2025-0974
基于LAMP-CRISPR/Cas12a技术快速检测转基因玉米DBN9501转化体特异性
Rapid Detection of Transgenic Maize DBN9501 Event-specificity Based on LAMP-CRISPR-Cas12a Technology
摘要
Abstract
[Objective]The event specificity is a key focus of transgenic regulation.Currently,laboratory testing remains the primary method for detecting event specificity.Therefore,it is an urgent need to develop a method that can be quickly detected on-site,thus meeting the regulatory needs for genetically modified organisms.[Method]This study used insect-resistant and herbicide-tolerant transgenic maize DBN9501 as the material,and utilized loop-mediated isothermal amplification(LAMP)combined with CRISPR/Cas12a technology,assisted by a lateral flow test strip display,to develop a rapid detection method suitable for on-site event-specificity.[Result]This detection method has a detection specificity.The limit of detection is about 20 copies,that is,0.1%event content,time of detection<60 min.[Conclusion]The LAMP-CRISPR/Cas method enables the rapid detection of maize DBN9501 event-specificity,providing technical support in supervising the safety of genetically modified organisms in China.关键词
转基因玉米/转化体特异性/环介导等温扩增/CRISPR/Cas/快速检测Key words
transgenic maize/event-specificity/LAMP/CRISPR/Cas12a/rapid detection引用本文复制引用
王晶,刘卓艳,张晓磊,刘宝海,关海涛,温洪涛..基于LAMP-CRISPR/Cas12a技术快速检测转基因玉米DBN9501转化体特异性[J].生物技术通报,2026,42(2):149-157,9.基金项目
国家市场监督管理总局重点实验室(谷物及制品质量与安全)自立课题(GWZL2025-008),科技创新2030—重大项目子课题(2022ZD040190803),黑龙江省农业科技创新跨越工程农业科技基础创新优青项目(CX22YQ08) （谷物及制品质量与安全）
