水生生物学报2026，Vol.50Issue(4)：42-49,8.DOI:10.3724/1000-3207.2025.2025.0250
盔形溞头盔发育相关基因的功能验证:基于RNA干扰与形态观测分析
FUNCTIONAL BERIFICATION OF GENES RELATED TO HELMET DEVELOP-MENT IN DAPHNIA GALEATA:BASED ON RNA INTERGERENCE AND MOTPHOLOGICAL OBSERVATION ANALYSIS
摘要
Abstract
In order to explore the molecular mechanism of helmet development in Daphnia galeata,this study investi-gated the expression of two genes related to helmet development(DgTrepsin and DgFerritin)and changes of helmet morphology in D.galeata under RNA interference.The results showed that at a 2%concentration of E.coli,compared with the EGFP group,the DgTrypsin-interference group exhibited significant reductions in helmet length,relative helmet length,and the ratio of helmet length to head width,accompanied by a 27.45%decrease in DgDrypsin expres-sion.However,no significant morphological or gene expression changes were observed in the DgFerritin-interference group at this concentration.When the concentration concentration of E.coli was increased to 5%,both the DgTrypsin and DgFerritin groups showed significant decreases in all three helmet morphology metrics,along with respective gene expression reductions of 55.96%and 40.39%,respectively.These results indicate that higher levels of RNA interfe-rence have a stronger inhibitory effect on the morphological changes and expression levels of the target genes.The findings demonstrate that DgTrypsin and DgFerritin genes are involved in the development of D.galeata helmet providing a valuable reference for further exploration of molecular mechanisms underlying the morphogenesis in Daph-nia.关键词
DgTrypsin基因/DgFerritin基因/头盔/盔形溞/RNA干扰Key words
DgTrypsin genes/DgFerritin genes/Helmet/Daphnia galeata/RNA interference分类
生物科学引用本文复制引用
朱豪,邓道贵,金齐德,尹熠辉,韩灵凤,王梓昕,刘佳虹,付林然..盔形溞头盔发育相关基因的功能验证:基于RNA干扰与形态观测分析[J].水生生物学报,2026,50(4):42-49,8.基金项目
国家自然科学基金(31870451和32001155)资助[Supported by the National Natural Science Foundation of China(31870451 and 32001155)] （31870451和32001155）
