水生生物学报2026，Vol.50Issue(4)：82-94,13.DOI:10.3724/1000-3207.2025.2025.0329
两种体色草鱼杂交F1代生长、肌肉营养成分及皮肤转录组比较分析
COMPARATIVE ANALYSIS OF GROWTH,MUSCLE NUTRIENT COMPOSITION,AND SKIN TRANSCRIPTOME IN F1 HYBRIDS OF CTENOPHARYNGODON IDELLA WITH TWO DIFFERENT BODY COLORS
摘要
Abstract
To investigate the growth performance,muscle quality,and expression characteristics of body color-related genes in the F1 generation of three grass carp(Ctenopharyngodon idella)families with two different body colors,this study established three F1 families:Ctenopharyngodon idella var.gold(♀)×C.idella var.gold(♂)(GG),C.idella var.gold(♀)×C.idella(♂)(GB),and C.idella(♀)×C.idella(♂)(BB),and systematically evaluated their growth traits,muscle quality,and skin transcriptome profiles through morphometric,nutritional,and RNA-seq analyses.Key findings revealed that:(1)The GG family exhibited significantly higher weight gain rate and specific growth rate compared to the BB and GB families(P<0.05),showing a trend of GG>BB>GB;(2)Muscle composition analysis revealed significantly higher moisture and lower crude fat content in the GG family(P<0.05),along with elevated levels of eicosapentaenoic acid(EPA)+docosahexaenoic acid(DHA),total amino acids(TAA),total essential amino acids(TEAA),and total delicious amino acids(TDAA)compared to BB and GB families(P<0.05);(3)Protein quality assessment indicated higher amino acid score(AAS),chemical score(CS),and essential amino acid index(EAAI)in GG family than those in BB and GB families;(4)transcriptome analysis identified differentially expressed genes(DEGs)between GG/BB and GB/BB,which were significantly enriched in GO terms such as ATP metabolic process,mononucleotide metabolic process,purine nucleoside monophosphate metabolic process,mitochondrion,heme binding,tetrapyrrole binding,and oxidoreductase activity,as well as pathways including oxidative phosphorylation and cell adhesion molecules.DEGs related to body color formation in grass carp,such as cdk4,mt-atp6,csf1r,xdh,and sox9b,were identified.In conclusion,the GG family exhibits superior growth performance and nutritional profile compared to the GB and BB families,and the identification of body color-related genes provides a scientific basis for deciphering the molecular mechanisms and advancing the selective breeding of golden grass carp.关键词
生长性能/营养成分/杂交/转录组/肌肉品质/体色/草鱼Key words
Growth performance/Nutritional components/Hybridization/Transcriptome/Muscle quality/Body color/Ctenopharyngodon idella分类
农业科技引用本文复制引用
黄文星,侯佳浩,刘文玉,韩莹莹,吴春林,范洪祥,龚佳奇,叶泽邦,赵大显..两种体色草鱼杂交F1代生长、肌肉营养成分及皮肤转录组比较分析[J].水生生物学报,2026,50(4):82-94,13.基金项目
国家重点研发计划(2023YFD2401800) （2023YFD2401800）
江西省名特优水产品产业技术体系(JXARS-15)资助[Supported by the National Key R&D Program of China(2023YFD2401800) （JXARS-15）
Jiangxi Province Famous,Special,and High-quality Aquatic Products Research System(JXARS-15)] （JXARS-15）
